Team:UNICAMP-Brazil/Notebooks/August 20

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(New page: {{:Team:UNICAMP-Brazil/inc_topo}} {{:Team:UNICAMP-Brazil/inc calendar}} __NOTOC__ ==''' YeastGuard '''== ==== Terminator biobrick ==== The colonies didn’t grow in the liquid LB media...)
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==''' YeastGuard '''==
==''' YeastGuard '''==
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==== Terminator biobrick ====
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====Recovering the biobricks====
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The colonies didn’t grow in the liquid LB media.
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==== RBS biobrick ====
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* The terminator colonies didn’t grow in the liquid LB medium.
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We performed miniprep to get purified RBS biobrick (Protocol 2)
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* We performed miniprep of the RBS inocule ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2]).
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''Taís''
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==''' ColiGuard '''==
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==== Lux S (BBa_K091109) enzymatic digestion  ====
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*<p style=”text-align:justify;”>We tried one more time the enzymatic digestion with ''Xba''I and ''Pst''I, but didn’t find the part again. We expected a 530 band size, but we just noticed a plasmid band. Something is wrong with this part.</p>
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[[Image:20 agosto.JPG‎ |center|‎]]
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''Luige''
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Latest revision as of 01:12, 22 October 2009

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YeastGuard

Recovering the biobricks

  • The terminator colonies didn’t grow in the liquid LB medium.
  • We performed miniprep of the RBS inocule (Protocol 2).

Taís

ColiGuard

Lux S (BBa_K091109) enzymatic digestion

  • We tried one more time the enzymatic digestion with XbaI and PstI, but didn’t find the part again. We expected a 530 band size, but we just noticed a plasmid band. Something is wrong with this part.

‎

Luige