Team:IBB Pune/Parts

From 2009.igem.org

(Difference between revisions)
(→Parts shipped to the registry)
 
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{{Team:IBB_Pune/header}}
{{Team:IBB_Pune/header}}
{{Team:IBB_Pune/menu}}
{{Team:IBB_Pune/menu}}
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<br><br>
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<p><span style="font-weight:bold; font-size:200%; color:#000000;">Parts shipped to the registry</span></p>
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[[Image:Truck.jpg|center| "width: 1400 px"]]
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=Parts shipped to the registry=
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{|class="wikitable sortable" border="1"
{|class="wikitable sortable" border="1"
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|- style="background: #1560BD; text-align: center;"
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|- style="background: #339933; text-align: center;"
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|width=15| '''Sr No.°'''
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|width=15| '''Sr No.'''
|width=85| '''BioBrick Name'''
|width=85| '''BioBrick Name'''
|width=160| '''Part Name'''
|width=160| '''Part Name'''
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|width=50| '''LENGTH (bp)'''
|width=50| '''LENGTH (bp)'''
|width=1| '''SEQUENCED'''
|width=1| '''SEQUENCED'''
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|- style="background:#99BADD; color:black; text-align: center"  
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|- style="background:#66ffcc; color:black; text-align: center"  
| 1
| 1
| [http://partsregistry.org/Part:BBa_K233306 BBa_K233306]   
| [http://partsregistry.org/Part:BBa_K233306 BBa_K233306]   
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| 123
| 123
| Yes
| Yes
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|- style="background:#99BADD; color:black; text-align: center"
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|- style="background:#66ffcc; color:black; text-align: center"
| 2
| 2
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233004 BBa_K233004]   
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233004 BBa_K233004]   
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| 30
| 30
| Yes
| Yes
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|- style="background:#99BADD; color:black; text-align: center"
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|- style="background:#66ffcc; color:black; text-align: center"
| 3
| 3
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233307 BBa_K233307]   
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233307 BBa_K233307]   
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| 139
| 139
| Yes
| Yes
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|- style="background:#99BADD; color:black; text-align: center"
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|- style="background:#66ffcc; color:black; text-align: center"
| 4
| 4
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233008 BBa_K233008]   
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233008 BBa_K233008]   
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| Signalling
| Signalling
| This construct is an intermediate in the production of an exportable GFP.
| This construct is an intermediate in the production of an exportable GFP.
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| pSB1AK3
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| [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
| 865
| 865
| -
| -
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|- style="background:#99BADD; color:black; text-align: center"
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|- style="background:#66ffcc; color:black; text-align: center"
| 5
| 5
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233309 BBa_K233309]   
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233309 BBa_K233309]   
Line 58: Line 56:
| Signalling
| Signalling
| This is an intermediate which can be used for constructing a module which can be used to produce an exportable GFP
| This is an intermediate which can be used for constructing a module which can be used to produce an exportable GFP
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| pSB1AK3
+
| [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
| 849
| 849
| -
| -
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|- style="background:#99BADD; color:black; text-align: center"
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|- style="background:#66ffcc; color:black; text-align: center"
| 6
| 6
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233310 BBa_K233310]   
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233310 BBa_K233310]   
Line 67: Line 65:
| Reporter
| Reporter
| Promoter-RBS-GFP- constitutive green fluorescent protein device
| Promoter-RBS-GFP- constitutive green fluorescent protein device
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| pSB1AK3
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| [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
| 800
| 800
| -
| -
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|- style="background:#99BADD; color:black; text-align: center"
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|- style="background:#66ffcc; color:black; text-align: center"
| 7
| 7
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233311 BBa_K233311]   
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233311 BBa_K233311]   
Line 76: Line 74:
| Device
| Device
| This device codes for an exportable GFP
| This device codes for an exportable GFP
-
| pSB1AK3
+
| [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
| 945
| 945
| -
| -
-
|- style="background:#99BADD; color:black; text-align: center"
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|- style="background:#66ffcc; color:black; text-align: center"
| 8
| 8
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233312 BBa_K233312]   
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233312 BBa_K233312]   
Line 85: Line 83:
| Device
| Device
| This device codes for an exportable GFP
| This device codes for an exportable GFP
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| pSB1AK3
+
| [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
| 929
| 929
| -
| -
-
|- style="background:#99BADD; color:black; text-align: center"
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|- style="background:#66ffcc; color:black; text-align: center"
| 9
| 9
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233313 BBa_K233313]   
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233313 BBa_K233313]   
Line 94: Line 92:
| Composite
| Composite
| construct designed to produce AHL (a quorum sensing molecule) in response to environmental AHL
| construct designed to produce AHL (a quorum sensing molecule) in response to environmental AHL
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| pSB1AK3
+
| [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
| 725
| 725
| -
| -
-
|- style="background:#99BADD; color:black; text-align: center"
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|- style="background:#66ffcc; color:black; text-align: center"
| 10
| 10
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233314 BBa_K233314]   
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233314 BBa_K233314]   
Line 103: Line 101:
| Generator
| Generator
| This part allows constitutive production of the LuxR protein which binds to AHL and the LuxR-AHL complex activates the pLuxR promoter.
| This part allows constitutive production of the LuxR protein which binds to AHL and the LuxR-AHL complex activates the pLuxR promoter.
-
| pSB1A2
+
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| 881
| 881
| -
| -
-
|- style="background:#99BADD; color:black; text-align: center"
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|- style="background:#66ffcc; color:black; text-align: center"
| 11
| 11
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233315 BBa_K233315]   
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233315 BBa_K233315]   
Line 112: Line 110:
| Generator
| Generator
|This part allows constitutive production of the LuxR protein  
|This part allows constitutive production of the LuxR protein  
-
| pSB1A2
+
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| 824
| 824
| -
| -
-
|- style="background:#99BADD; color:black; text-align: center"
+
|- style="background:#66ffcc; color:black; text-align: center"
|12
|12
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K233316 BBa_K233316]
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K233316 BBa_K233316]
Line 121: Line 119:
|Device
|Device
|This part can be used to calibrate the working of the lac operator
|This part can be used to calibrate the working of the lac operator
-
|pSB1A2
+
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
|73
|73
| -
| -
-
|- style="background:#99BADD; color:black; text-align: center"
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|- style="background:#66ffcc; color:black; text-align: center"
|13
|13
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K233317 BBa_K233317]
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K233317 BBa_K233317]
Line 130: Line 128:
|measurement
|measurement
|This part can be used to calibrate the working of BBa_J13002
|This part can be used to calibrate the working of BBa_J13002
-
|pSB1A2
+
|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|912
|912
| -  
| -  
 +
|-style="background:#66ffcc; color:black; text-align: center"
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| 14
 +
| [http://partsregistry.org/Part:BBa_K233003 BBa_K233003] 
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| AND Gate 1
 +
| Regulatory
 +
| This is an AND Gate, responsive to two external inputs, Lactose and AHL.
 +
| [http://partsregistry.org/Part:pSB1C3 pSB1C3]
 +
| 94
 +
| -
|}
|}
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<br>
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=Parts designed by us=
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<p><span style="font-weight:bold; font-size:200%; color:#000000;">Parts designed by us</span></p><br>
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1) <html>
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{|class="wikitable sortable" border="1"
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<a href="http://partsregistry.org/Part:BBa_K233004"> Part: BBa_K233004</a>
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|- style="background: #339933; Align: center;"
-
</html> We have successfully obtained and shipped this part. It is the lactose operator induced by lactose or IPTG. This part is an  integral cog in our AND gate.
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|width=100| '''Sr No.'''
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|width=200| '''BioBrick Name'''
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2)<html>
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|width=400| '''Part Name'''
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<a href="http://partsregistry.org/Part:BBa_BBa_K233306"> Part: BBa_K233306</a>
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|WIDTH=400|'''Description'''
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</html>We have successfully obtained and shipped this part. YcdB is one of our two Export Tags.
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|-
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|- style="background:#66ffcc; color:black; text-align: center"
-
3)<html>
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|1
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<a href="http://partsregistry.org/Part:BBa_K233307"> Part: BBa_K233307</a>
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|<html> <a href="http://partsregistry.org/Part:BBa_K233002"> Part: BBa_K233002</a> </html>  
-
</html>We have successfully obtained and shipped this part. TorA is the second of our Export Tags.
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|Snowdrift 2 , using YcdB.  
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|A module to secrete B-galactosidase using the TAT-export Pathway
-
4) <html>
+
|-
-
<a href="http://partsregistry.org/Part:BBa_K233008"> Part: BBa_K233008</a>
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|- style="background:#66ffcc; color:black; text-align: center"
-
</html> We have successfully obtained and shipped this part. It is a GFP secretion device.
+
|2
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+
|<html> <a href="http://partsregistry.org/Part:BBa_K233001"> Part: BBa_K233001</a>
-
5)<html>
+
</html>
-
<a href="http://partsregistry.org/Part:BBa_K233309"> Part: BBa_K233309</a>
+
|Snowdrift 1 , using TorA
-
</html>We have successfully obtained and shipped this part. It is a GFP secretion device.
+
| A module to secrete B-galactosidase using the TAT-export Pathway
-
 
+
|-
-
6)<html>
+
|- style="background:#66ffcc; color:black; text-align: center"
-
<a href="http://partsregistry.org/Part:BBa_K233312"> Part: BBa_K233312</a>
+
|3
-
</html>We have successfully obtained and shipped this part. It is GFP secretion device.
+
| <html>
-
 
+
-
7)<html>
+
-
<a href="http://partsregistry.org/Part:BBa_K233310"> Part: BBa_K233310</a>
+
-
</html>We have successfully obtained and shipped this part. It is a constitutive green fluorescent protein device
+
-
 
+
-
8)<html>
+
-
<a href="http://partsregistry.org/Part:BBa_K233311"> Part: BBa_K233311</a>
+
-
</html>We have successfully obtained and shipped this part. It is a Protein(GFP) secretion device.
+
-
 
+
-
9)<html>
+
-
<a href="http://partsregistry.org/Part:BBa_K233313"> Part: BBa_K233313</a>
+
-
</html>We have successfully obtained and shipped this part. It is an AHL induced AHL producer
+
-
 
+
-
10)<html>
+
-
<a href="http://partsregistry.org/Part:BBa_K233314"> Part: BBa_K233314</a>
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-
</html>We have successfully obtained and shipped this part. It is a constitutive LuxR production device
+
-
 
+
-
11)<html>
+
-
<a href="http://partsregistry.org/Part:BBa_K233315"> Part: BBa_K233315</a>
+
-
</html>We have successfully obtained and shipped this part. It is a constitutive LuxR generating device
+
-
 
+
-
12)<html>
+
-
<a href="http://partsregistry.org/Part:BBa_K233316"> Part: BBa_K233316</a>
+
-
</html>We have successfully obtained and shipped this part. It is an intermediate part for our AND gate, helping us characterise the AND gates.
+
-
 
+
-
13)<html>
+
-
<a href="http://partsregistry.org/Part:BBa_K233317"> Part: BBa_K233317</a>
+
-
</html>We have successfully obtained and shipped this part. It is an RFP measurement device
+
-
 
+
-
14) <html>
+
-
<a href="http://partsregistry.org/Part:BBa_K233002"> Part: BBa_K233002</a>
+
-
</html> Still in the design stage. We will try to create this DNA part in our lab.
+
-
 
+
-
15) <html>
+
-
<a href="http://partsregistry.org/Part:BBa_K233003"> Part: BBa_K233003</a>
+
-
</html> Still in the design stage. We will try to create this DNA part in our lab.
+
-
 
+
-
16) <html>
+
-
<a href="http://partsregistry.org/Part:BBa_K233001"> Part: BBa_K233001</a>
+
-
</html> Still in the design stage. We will try to create this DNA part in our lab.
+
-
 
+
-
17) <html>
+
<a href="http://partsregistry.org/Part:BBa_K233005"> Part: BBa_K233005</a>
<a href="http://partsregistry.org/Part:BBa_K233005"> Part: BBa_K233005</a>
-
</html> Still in the design stage. We will try to create this DNA part in our lab.
+
</html>
-
 
+
|AND gate 2
-
18) <html>
+
| phage protein ogr regulated AND GATE
 +
|-
 +
|- style="background:#66ffcc; color:black; text-align: center"
 +
|4
 +
|<html>
<a href="http://partsregistry.org/Part:BBa_K233006"> Part: BBa_K233006</a>
<a href="http://partsregistry.org/Part:BBa_K233006"> Part: BBa_K233006</a>
-
</html> Still in the design stage. We will try to create this DNA part in our lab.
+
</html>  
 +
|AND Gate 3
 +
|Phage T7 polymerase regulated And Gate
 +
|-
 +
|- style="background:#66ffcc; color:black; text-align: center"
 +
|5
 +
|<html>
 +
<a href="http://partsregistry.org/Part:BBa_K233318"> Part: BBa_K233318</a>
 +
</html>
 +
|Gfp exporter 1
 +
|A tester for Snowdrift 1
 +
|-
 +
|- style="background:#66ffcc; color:black; text-align: center"
 +
|6
 +
|<html>
 +
<a href="http://partsregistry.org/Part:BBa_K233319"> Part: BBa_K233319</a>
 +
</html>
 +
|Gfp exporter 2
 +
|A tester for Snowdrift 2
 +
|-
 +
|- style="background:#66ffcc; color:black; text-align: center"
 +
|7
 +
|<html>
 +
<a href="http://partsregistry.org/Part:BBa_K233320"> Part: BBa_K233320</a>
 +
</html>
 +
|Turing Machine- Unary Adder
 +
|The simplified construct for the Turing machine
 +
|-
 +
|- style="background:#66ffcc; color:black; text-align: center"
 +
|8
 +
|<html>
 +
<a href="http://partsregistry.org/Part:BBa_K233321"> Part: BBa_K233321</a>
 +
</html>
 +
|Turing Machine- Unary Adder 2
 +
|The simplified construct for the Turing Machine 2
 +
|-
 +
|- style="background:#66ffcc; color:black; text-align: center"
 +
|9
 +
|<html>
 +
<a href="http://partsregistry.org/Part:BBa_K233322"> Part: BBa_K233322</a>
 +
</html>
 +
|Turing machine cassette 2
 +
|AHL and Lactose Modulated Cell Lysis
 +
|-
 +
|- style="background:#66ffcc; color:black; text-align: center"
 +
|10
 +
|<html>
 +
<a href="http://partsregistry.org/Part:BBa_K233323"> Part: BBa_K233323</a>
 +
</html>
 +
|Turing machine cassette 2
 +
|AHL and Lactose modulated Gfp production
 +
|-
 +
|- style="background:#66ffcc; color:black; text-align: center"
 +
|11
 +
|<html>
 +
<a href="http://partsregistry.org/Part:BBa_K233324"> Part: BBa_K233324</a>
 +
</html>
 +
|pIDTSmart-KAN Vector
 +
|The Backbone of our favorite parts
 +
|-style="background:#66ffcc; color:black; text-align: center"
 +
|12
 +
|<html>
 +
<a href="http://partsregistry.org/Part:BBa_K233325"> Part: BBa_K233325</a>
 +
</html>
 +
|The Complete Turing Machine Construct
 +
|The Complete Turing Machine Construct
 +
|-style="background:#66ffcc; color:black; text-align: center"
 +
|}

Latest revision as of 01:29, 22 October 2009






Parts shipped to the registry

"width: 1400 px"
Sr No. BioBrick Name Part Name TYPE Description Backbone LENGTH (bp) SEQUENCED
1 [http://partsregistry.org/Part:BBa_K233306 BBa_K233306] YcdB Signalling YcdB - This part is a export tag that utilizes the Twin Arginine Transport pathway(TAT) [http://partsregistry.org/Part:BBa_K233324 BBa_K233324] 123 Yes
2 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233004 BBa_K233004] lac operator regulatory Lac operator. [http://partsregistry.org/Part:BBa_K233324 BBa_K233324] 30 Yes
3 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233307 BBa_K233307] TorA Signalling TorA- This part is a export tag that utilizes the Twin Arginine Transport pathway(TAT) [http://partsregistry.org/Part:BBa_K233324 BBa_K233324] 139 Yes
4 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233008 BBa_K233008] TorA-GFP Signalling This construct is an intermediate in the production of an exportable GFP. [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] 865 -
5 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233309 BBa_K233309] YcdB-GFP Signalling This is an intermediate which can be used for constructing a module which can be used to produce an exportable GFP [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] 849 -
6 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233310 BBa_K233310] constitutive GFP device Reporter Promoter-RBS-GFP- constitutive green fluorescent protein device [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] 800 -
7 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233311 BBa_K233311] Protein(GFP) secretion device Device This device codes for an exportable GFP [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] 945 -
8 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233312 BBa_K233312] GFP secretion device using Ycdb Device This device codes for an exportable GFP [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] 929 -
9 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233313 BBa_K233313] ahl induced ahl producer Composite construct designed to produce AHL (a quorum sensing molecule) in response to environmental AHL [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] 725 -
10 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233314 BBa_K233314] constitutive luxR production device Generator This part allows constitutive production of the LuxR protein which binds to AHL and the LuxR-AHL complex activates the pLuxR promoter. [http://partsregistry.org/Part:pSB1A2 pSB1A2] 881 -
11 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233315 BBa_K233315] constitutive LuxR generating device Generator This part allows constitutive production of the LuxR protein [http://partsregistry.org/Part:pSB1A2 pSB1A2] 824 -
12 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233316 BBa_K233316] Constitutive promoter lac operator Device This part can be used to calibrate the working of the lac operator [http://partsregistry.org/Part:pSB1A2 pSB1A2] 73 -
13 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233317 BBa_K233317] rfp measurement device measurement This part can be used to calibrate the working of BBa_J13002 [http://partsregistry.org/Part:pSB1A2 pSB1A2] 912 -
14 [http://partsregistry.org/Part:BBa_K233003 BBa_K233003] AND Gate 1 Regulatory This is an AND Gate, responsive to two external inputs, Lactose and AHL. [http://partsregistry.org/Part:pSB1C3 pSB1C3] 94 -


Parts designed by us


Sr No. BioBrick Name Part Name Description
1 Part: BBa_K233002 Snowdrift 2 , using YcdB. A module to secrete B-galactosidase using the TAT-export Pathway
2 Part: BBa_K233001 Snowdrift 1 , using TorA A module to secrete B-galactosidase using the TAT-export Pathway
3 Part: BBa_K233005 AND gate 2 phage protein ogr regulated AND GATE
4 Part: BBa_K233006 AND Gate 3 Phage T7 polymerase regulated And Gate
5 Part: BBa_K233318 Gfp exporter 1 A tester for Snowdrift 1
6 Part: BBa_K233319 Gfp exporter 2 A tester for Snowdrift 2
7 Part: BBa_K233320 Turing Machine- Unary Adder The simplified construct for the Turing machine
8 Part: BBa_K233321 Turing Machine- Unary Adder 2 The simplified construct for the Turing Machine 2
9 Part: BBa_K233322 Turing machine cassette 2 AHL and Lactose Modulated Cell Lysis
10 Part: BBa_K233323 Turing machine cassette 2 AHL and Lactose modulated Gfp production
11 Part: BBa_K233324 pIDTSmart-KAN Vector The Backbone of our favorite parts
12 Part: BBa_K233325 The Complete Turing Machine Construct The Complete Turing Machine Construct
Retrieved from "http://2009.igem.org/Team:IBB_Pune/Parts"