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Team:UNICAMP-Brazil/Notebooks/August 28
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====''Kluyveromices lactis'' genes==== | ====''Kluyveromices lactis'' genes==== | ||
| - | The PCR for the ''K. lactis'' genes worked very well! =] We got specific bands for the 3 sequences. | + | *<p style=”text-align:justify;”>The PCR for the ''K. lactis'' genes worked very well! =] We got specific bands for the 3 sequences (agarose gel above).</p> |
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====Terminator biobrick==== | ====Terminator biobrick==== | ||
| - | * Colony PCR | + | *<p style=”text-align:justify;”>Colony PCR</p> |
The colony PCR was made to confirm the presence of terminator transformants, because the antibiotic resistence is not working to any antibiotics. | The colony PCR was made to confirm the presence of terminator transformants, because the antibiotic resistence is not working to any antibiotics. | ||
| - | We plated the cells in LB and picked up some colonies with a toothpick, inoculated on a backup LB plate and put the toothpick on a tube with 20µl H2O | + | [[Image:PCRKL.jpg|350px|center]] |
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| + | We plated the cells in LB and picked up some colonies with a toothpick, inoculated on a backup LB plate and put the toothpick on a tube with 20µl H2O. Then, denatured these cells at 95°C during 5 minutes and heat shocked on ice for 5 minutes. Spinned the tubes and used 1 µl to make the PCR. | ||
| - | * Preparing for Miniprep | + | *<p style=”text-align:justify;”>Preparing for Miniprep</p> |
| - | We also inoculated | + | We also inoculated 10 colonies transformed with the terminator biobrick to perform the miniprep tomorrow. |
''Wesley'' | ''Wesley'' | ||
{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} | ||
Latest revision as of 01:50, 22 October 2009
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