Team:Kyoto/CiC/Notebook/0921-0925

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==0914-0918 : what to do. what to do. what to do. what to do. ==
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==0914-0918 : constructing sig-GFP generator,sig-EGFP generator(HeLa)  ==
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===Monday, 21 September===
===Monday, 21 September===
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**miniprep
**miniprep
*sig-EGFP generator(HeLa)
*sig-EGFP generator(HeLa)
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**PEGFP-N3 VECTOR (EGFP generator)
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**Restriction Enzyme PEGFP-N3 VECTOR (EGFP generator)
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**hosphorylation signal sequence primer
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**annering signal sequence primer
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====Results====
 
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*消えた
 
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!NO.!!Sample name!!conc./(ng/ul)!!conc.(ul)
!NO.!!Sample name!!conc./(ng/ul)!!conc.(ul)
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|-
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|1||pEGFP-N3(EcoR1,Xho1)(||224.3
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|1||pEGFP-N3(EcoR1,Xho1)||224.3||1
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|2||signal sequence(||38.1
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|2||signal sequence||0.3||11
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=====Transformation=====
=====Transformation=====
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No colony was observed (090917 10:00)　
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No colony was observed. (090917 10:00)　
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Latest revision as of 02:06, 22 October 2009

  1. Home
  2. CiC
  3. Notebook

0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

0914-0918 : constructing sig-GFP generator,sig-EGFP generator(HeLa)

Monday, 21 September

To Do

  • HIV-TAT-(LALAAAA)3-generator
    • Colony PCR

Results

  • Binds of expected size were observed.

Tuesday, 22 September

To Do

  • HIV-TAT-(LALAAAA)3-generator
    • miniprep
  • sig-EGFP generator(HeLa)
    • Restriction Enzyme PEGFP-N3 VECTOR (EGFP generator)
    • hosphorylation signal sequence primer
    • annering signal sequence primer

Wednesday, 23 September

To Do

  • sig-EGFP generator
    • ligation
    • transformation

Results

ligation
conc.
NO.Sample nameconc./(ng/ul)conc.(ul)
1pEGFP-N3(EcoR1,Xho1)224.31
2signal sequence0.311
Transformation

No colony was observed. (090917 10:00)　

Thursday, 17 September

To Do

  • Make repetitive sequence (MPR)
  • Transformation(retry)

Retrieved from "http://2009.igem.org/Team:Kyoto/CiC/Notebook/0921-0925"