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Team:Kyoto/CiC/Notebook/0921-0925
From 2009.igem.org
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**annering signal sequence primer | **annering signal sequence primer | ||
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=====Transformation===== | =====Transformation===== | ||
| - | No colony was observed (090917 10:00) | + | No colony was observed. (090917 10:00) |
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Latest revision as of 02:06, 22 October 2009
- 0907-0911
- constructing HIV-TAT-(LALAAAA)3-generator
- 0914-0918
- constructing HIV-TAT-(LALAAAA)3-generator
- 0921-0925
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 0928-1002
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1005-1009
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1012-1016
- Observation
- 1019-1023
- Observation
0914-0918 : constructing sig-GFP generator,sig-EGFP generator(HeLa)
Monday, 21 September
To Do
- HIV-TAT-(LALAAAA)3-generator
- Colony PCR
Results
- Binds of expected size were observed.
Tuesday, 22 September
To Do
- HIV-TAT-(LALAAAA)3-generator
- miniprep
- sig-EGFP generator(HeLa)
- Restriction Enzyme PEGFP-N3 VECTOR (EGFP generator)
- hosphorylation signal sequence primer
- annering signal sequence primer
Wednesday, 23 September
To Do
- sig-EGFP generator
- ligation
- transformation
Results
ligation
| NO. | Sample name | conc./(ng/ul) | conc.(ul) |
|---|---|---|---|
| 1 | pEGFP-N3(EcoR1,Xho1) | 224.3 | 1 |
| 2 | signal sequence | 0.3 | 11 |
Transformation
No colony was observed. (090917 10:00)
Thursday, 17 September
To Do
- Make repetitive sequence (MPR)
- Transformation(retry)
