Team:UNICAMP-Brazil/Notebooks/September 3

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====Electrocompetent ''E. coli''====
====Electrocompetent ''E. coli''====
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*<p style=”text-align:justify;”>Today we prepared solutions, materials and the pre inoculum to prepare electrocompetent ''E. coli''.</p>
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*<p style=”text-align:justify;”>Today we prepared solutions, materials and the pre-inoculum to prepare electrocompetent ''E. coli''.</p>
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=='''Coliguard'''==
====Hemolysin Operon Amplification====
====Hemolysin Operon Amplification====
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*<p style=”text-align:justify;”>We did the PCR to amplify the hemolysin operon using the total DNA extration that we had made before. Our sequence have almost 4000bp so we used a extension time of 3 minutes, the annelling temperatures in Celsius degrees was 56, 54, 52 and 51. No DNA fragment was shown in the agarose gel. .</p>
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*<p style=”text-align:justify;”>We did the PCR to amplify the hemolysin operon using the total DNA extration that we had made before. Our sequence have almost 4000bp so we used a extension time of 3 minutes, the annelling temperatures in Celsius degrees was 56, 54, 52 and 51. No DNA fragment was shown in the agarose gel.</p>
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''Marcos''
==''' YeastGuard '''==
==''' YeastGuard '''==
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====Terminator Biobrick====
====Terminator Biobrick====
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*<p style=”text-align:justify;”>The terminator plasmid was quantified and we made another digestion, Protocol X (see protocols section). We found by electrophoresis that it worked!</p>
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*<p style=”text-align:justify;”>The terminator plasmid was quantified and we made another digestion, [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Digestion_reaction Protocol 14]. We found by electrophoresis that it worked!</p>
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[[image:end.png|150px|center]]
''Wesley''
''Wesley''
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Latest revision as of 02:06, 22 October 2009

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MicroGuards

Electrocompetent E. coli

  • Today we prepared solutions, materials and the pre-inoculum to prepare electrocompetent E. coli.

Coliguard

Hemolysin Operon Amplification

  • We did the PCR to amplify the hemolysin operon using the total DNA extration that we had made before. Our sequence have almost 4000bp so we used a extension time of 3 minutes, the annelling temperatures in Celsius degrees was 56, 54, 52 and 51. No DNA fragment was shown in the agarose gel.

Marcos

YeastGuard

Terminator Biobrick

  • The terminator plasmid was quantified and we made another digestion, Protocol 14. We found by electrophoresis that it worked!

End.png

Wesley




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