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Team:UNICAMP-Brazil/Notebooks/September 3
From 2009.igem.org
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====Electrocompetent ''E. coli''==== | ====Electrocompetent ''E. coli''==== | ||
| - | *<p style=”text-align:justify;”>Today we prepared solutions, materials and the pre inoculum to prepare electrocompetent ''E. coli''.</p> | + | *<p style=”text-align:justify;”>Today we prepared solutions, materials and the pre-inoculum to prepare electrocompetent ''E. coli''.</p> |
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| + | =='''Coliguard'''== | ||
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| + | ====Hemolysin Operon Amplification==== | ||
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| + | *<p style=”text-align:justify;”>We did the PCR to amplify the hemolysin operon using the total DNA extration that we had made before. Our sequence have almost 4000bp so we used a extension time of 3 minutes, the annelling temperatures in Celsius degrees was 56, 54, 52 and 51. No DNA fragment was shown in the agarose gel.</p> | ||
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| + | ''Marcos'' | ||
==''' YeastGuard '''== | ==''' YeastGuard '''== | ||
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====Terminator Biobrick==== | ====Terminator Biobrick==== | ||
| - | *<p style=”text-align:justify;”>The terminator plasmid was quantified and we made another digestion, Protocol | + | *<p style=”text-align:justify;”>The terminator plasmid was quantified and we made another digestion, [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Digestion_reaction Protocol 14]. We found by electrophoresis that it worked!</p> |
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| + | [[image:end.png|150px|center]] | ||
''Wesley'' | ''Wesley'' | ||
{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} | ||
Latest revision as of 02:06, 22 October 2009
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