Team:UNICAMP-Brazil/Notebooks/September 9

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*<p style=”text-align:justify;”>Today we digested the JEN1 ORF again with the enzymes ''Xba''I and ''Spe''I (according to Protocol X). The agarose gel confirmed that it worked!</p>
*<p style=”text-align:justify;”>Today we digested the JEN1 ORF again with the enzymes ''Xba''I and ''Spe''I (according to Protocol X). The agarose gel confirmed that it worked!</p>
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[[Image:20090909_UNICAMP-Brasil_GEL_Yeastguard_1.png|200px|center]]
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[[Image:20090909_UNICAMP-Brasil_GEL_Yeastguard_1.png|100px|center]]
*<p style=”text-align:justify;”>Expected band length: 1873pb.</p>
*<p style=”text-align:justify;”>Expected band length: 1873pb.</p>

Revision as of 02:20, 22 October 2009

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YeastGuard

Lactate Permease (JEN1 ORF)

  • Today we digested the JEN1 ORF again with the enzymes XbaI and SpeI (according to Protocol X). The agarose gel confirmed that it worked!

20090909 UNICAMP-Brasil GEL Yeastguard 1.png

  • Expected band length: 1873pb.

  • We also confirmed the PCR of Lysozyme and its digestion.

Taís




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