Team:UNICAMP-Brazil/Notebooks/September 14
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(→PY Promoter - Purification of the digestion reactions) |
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====Culture growth of bacteria containing the F plasmid==== | ====Culture growth of bacteria containing the F plasmid==== | ||
- | *<p style=”text-align:justify;”>From a permanent culture of bacteria | + | *<p style=”text-align:justify;”>From a permanent culture of bacteria containing the F plasmid, a sample was inoculated into liquid LB-AMP medium and let grow O/N at 37°C.</p> |
''Gabriel'' | ''Gabriel'' | ||
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====finO and finP's transformed bacteria==== | ====finO and finP's transformed bacteria==== | ||
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*<p style=”text-align:justify;”>We purified the digestion reactions of PY1 and PY2 with the Invitrogen's PureLink™ PCR Purification Kit, following the manufacturer's protocol without modifications.</p> | *<p style=”text-align:justify;”>We purified the digestion reactions of PY1 and PY2 with the Invitrogen's PureLink™ PCR Purification Kit, following the manufacturer's protocol without modifications.</p> | ||
- | *<p style=”text-align:justify;”>We also prepared an agarose gel to run the digestion reaction of BBa_J23100. The band in the gel | + | *<p style=”text-align:justify;”>We also prepared an agarose gel to run the digestion reaction of BBa_J23100. The band in the gel had the expected size (2940 bp). However, it is not possible to confirm the digestion by the size in the gel because the difference between the plasmid BBa_J23100 with and without the fragment excised by ''Xba''I and ''Spe''I is too small.</p> |
[[Image:py_gel_2.png|200px|center]] | [[Image:py_gel_2.png|200px|center]] |
Latest revision as of 02:35, 22 October 2009
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