Team:UNICAMP-Brazil/Notebooks/September 18
From 2009.igem.org
(→PY Promoter - Miniprep and PCR confirmation) |
(→CeaB, CeiB and BBa_B0015 restriction, purification and quantification) |
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==== CeaB, CeiB and BBa_B0015 restriction, purification and quantification ==== | ==== CeaB, CeiB and BBa_B0015 restriction, purification and quantification ==== | ||
- | *<p style=”text-align:justify;”>We made the DNA purification in order to clean all buffer PCR salts. Immediately, we cut CeaB and CeiB purified with | + | *<p style=”text-align:justify;”>We made the DNA purification in order to clean all buffer PCR salts. Immediately, we cut CeaB and CeiB purified with ''Spe''I and ''Xba''I restriction enzymes. We made that, because we want to join the CeaB DNA with a terminator. The terminator chosen was BBa_B0015. Separately, we cut the terminator plasmid with ''Xba''I enzyme restriction and quantificated DNA. Then, we performed DNA purification with Pure-link Purification PCR kit.</p> |
''Luige'' | ''Luige'' | ||
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====Cre-Recombinase and pSB1A3 digestion==== | ====Cre-Recombinase and pSB1A3 digestion==== |
Revision as of 02:45, 22 October 2009
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