Team:Kyoto/CiC/Notebook

From 2009.igem.org

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#[[Team:Kyoto|Home]]
#[[Team:Kyoto|Home]]
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#[[Team:Kyoto/CiC|Cells in Cells]]
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#[[Team:Kyoto/CiC|CiC]]
#Notebook
#Notebook
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===Monday, June 9===
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</div><!-- /#rightcontents --></html>
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====To Do on 24 July====
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*Miniprep (3-O-2, 3-A-4)
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*Restriction Enzyme Digestion
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*Electrophoresis
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====Results====
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=====Miniprep=====
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*3-O-2, 3-A-4 was minipreped.
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=====Digestion=====
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*Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
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{| border="1" style="text-align:center;width:50%"
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|+Sample list
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!NO.!!Sample Name!!Enzyme
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|-align="center"
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|1||3-O-2||Eco R1, PST1
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|-align="center"
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|2||3-A-4||Eco R1, PST1
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|-align="center"
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|3||3-O-2||Eco R1
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|-align="center"
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|4||3-A-4||Eco R1
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|}
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=====Electrophoresis=====
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[[Image:Kyoto_e_090724_1.png|250px]]
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* Very clear bands were found. (See the gel image in right!)
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* The bands are listed below.
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{| border="1" style="text-align:center;width:50%"
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|+Parts and Plasmid length
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!!!3-O-2!!3-A-4
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|-
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|Parts length / bp||2837||487
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|-
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|Plasmid length / bp||3266||3266
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|}
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{{:Team:Kyoto/Pad_between_dates}}
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===Tuseday, June 10===
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====To Do on 24 July====
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*Miniprep (3-O-2, 3-A-4)
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*Restriction Enzyme Digestion
+
-
*Electrophoresis
+
-
 
+
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====Results====
+
-
=====Miniprep=====
+
-
*3-O-2, 3-A-4 was minipreped.
+
-
 
+
-
=====Digestion=====
+
-
*Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
+
-
 
+
-
{| border="1" style="text-align:center;width:50%"
+
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|+Sample list
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!NO.!!Sample Name!!Enzyme
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|-align="center"
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|1||3-O-2||Eco R1, PST1
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|-align="center"
+
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|2||3-A-4||Eco R1, PST1
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|-align="center"
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|3||3-O-2||Eco R1
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|-align="center"
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|4||3-A-4||Eco R1
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|}
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+
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=====Electrophoresis=====
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[[Image:Kyoto_e_090724_1.png|250px]]
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* Very clear bands were found. (See the gel image in right!)
+
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* The bands are listed below.
+
-
 
+
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{| border="1" style="text-align:center;width:50%"
+
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|+Parts and Plasmid length
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!!!3-O-2!!3-A-4
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|-
+
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|Parts length / bp||2837||487
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|-
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|Plasmid length / bp||3266||3266
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|}
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{{:Team:Kyoto/Pad_endof_dates}}
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<html></div><!-- /#rightcontents --></html>
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Latest revision as of 02:58, 22 October 2009

0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

Retrieved from "http://2009.igem.org/Team:Kyoto/CiC/Notebook"