Team:UNICAMP-Brazil/Notebooks/September 9

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====Lactate Permease  (JEN1 ORF)====
====Lactate Permease  (JEN1 ORF)====
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Today we digested the JEN1 ORF again with the enzymes Xba1 and Spe1. The agarose gel confirmed that it worked!
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*<p style=”text-align:justify;”>Today we digested the ''JEN1'' ORF again with the enzymes ''Xba''I and ''Spe''I (according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Digestion_reaction Protocol 14]). The agarose gel confirmed that it worked!</p>
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GEL
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[[Image:20090909_UNICAMP-Brasil_GEL_Yeastguard_1.png|100px|center]]
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*<p style=”text-align:justify;”>Expected band length: 1873pb.</p>
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*<p style=”text-align:justify;”>We also confirmed the PCR of Lysozyme and its digestion.</p>
''Taís''
''Taís''
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Latest revision as of 03:38, 22 October 2009

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YeastGuard

Lactate Permease (JEN1 ORF)

  • Today we digested the JEN1 ORF again with the enzymes XbaI and SpeI (according to Protocol 14). The agarose gel confirmed that it worked!

20090909 UNICAMP-Brasil GEL Yeastguard 1.png

  • Expected band length: 1873pb.

  • We also confirmed the PCR of Lysozyme and its digestion.

Taís




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