Team:UNICAMP-Brazil/Notebooks/September 18
From 2009.igem.org
(→New biobricks - Second screening) |
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*<p style=”text-align:justify;”>Today we digested the purified PCR product for Cre-Recombinase without ATG start codon (see Septermber 13th) with ''Xba''I and ''Spe''I restriction enzymes. We also digested the vector pSB1A3 with the same enzymes, aiming in the future ligation between them.</p> | *<p style=”text-align:justify;”>Today we digested the purified PCR product for Cre-Recombinase without ATG start codon (see Septermber 13th) with ''Xba''I and ''Spe''I restriction enzymes. We also digested the vector pSB1A3 with the same enzymes, aiming in the future ligation between them.</p> | ||
- | *<p style=”text-align:justify;”>Digestion lasted 3 hours on both cases. | + | *<p style=”text-align:justify;”>Digestion lasted 3 hours on both cases.</p> |
''Víctor'' | ''Víctor'' | ||
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''Taís'' | ''Taís'' | ||
- | *<p style=”text-align:justify;”>We decided to make a bigger screening of our colonies to find the ones containing the biobricks (pJen1, pDLD, Lysozyme, JEN1 orf). We grew 24 colonies from each biobrick O.N. in liquid medium.</p> | + | *<p style=”text-align:justify;”>We decided to make a bigger screening of our colonies to find the ones containing the biobricks (pJen1, pDLD, Lysozyme, ''JEN1'' orf). We grew 24 colonies from each biobrick O.N. in liquid medium.</p> |
''Raíssa'' | ''Raíssa'' | ||
{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} |
Latest revision as of 03:42, 22 October 2009
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