Team:UNICAMP-Brazil/Notebooks/October 4
From 2009.igem.org
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(→Transformation of the BBa K112806 + BBa B0015 ligation) |
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====Cre-Recombinase without ATG==== | ====Cre-Recombinase without ATG==== | ||
*<p style=”text-align:justify;”>The colonies grew up well! All of them were transparent.</p> | *<p style=”text-align:justify;”>The colonies grew up well! All of them were transparent.</p> | ||
- | *<p style=”text-align:justify;”>We inoculated twenty colonies in liquid LB- | + | *<p style=”text-align:justify;”>We inoculated twenty colonies in liquid LB-AMP media in order to make a miniprep tomorrow.</p> |
- | *<p style=”text-align:justify;">We let then grow for overnight at 37°C. | + | *<p style=”text-align:justify;">We let then grow for overnight at 37°C.</p> |
''Victor'' | ''Victor'' | ||
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====Transformation of the BBa K112806 + BBa B0015 ligation==== | ====Transformation of the BBa K112806 + BBa B0015 ligation==== | ||
- | *<p style=”text-align:justify;”>We transformed the ligation made yesterday by | + | *<p style=”text-align:justify;”>We transformed the ligation made yesterday by electroporation according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Electroporation Protocol 3]</p> |
''Léo'' | ''Léo'' | ||
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==''' YeastGuard '''== | ==''' YeastGuard '''== | ||
- | ====New | + | ====[https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/pGEMStrategy New Strategy: pGEM]==== |
*<p style=”text-align:justify;”>Only the plates containing ''E. coli'' transformed with pDLD and Lysozyme showed colonies. Tomorrow we will make colony PCR to collect the right ones.</p> | *<p style=”text-align:justify;”>Only the plates containing ''E. coli'' transformed with pDLD and Lysozyme showed colonies. Tomorrow we will make colony PCR to collect the right ones.</p> |
Latest revision as of 03:46, 22 October 2009
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