EPF-Lausanne/20 July 2009

From 2009.igem.org

(Difference between revisions)
(New page: ===Wet Lab=== As the transformation of Saturday didn't work, we think that the plasmid Term BBa_B0010 has a problem. We tried to begin all over with 4 other Terminator from the registery. ...)
(→People in the lab)
 
(4 intermediate revisions not shown)
Line 1: Line 1:
-
===Wet Lab===
+
{{EPF-Lausanne09}}
 +
<div CLASS="epfltrick">__TOC__
 +
</div><div CLASS="epfl09">
 +
 
 +
<html>
 +
<body>
 +
<form action="input_button.htm">
 +
<p align="right">
 +
<input type="button" name="lien" value="18 July 2009"
 +
onClick="self.location.href='https://2009.igem.org/EPF-Lausanne/18_July_2009'">
 +
<input type="button" name="lien" value="21 July 2009"
 +
onClick="self.location.href='https://2009.igem.org/EPF-Lausanne/21_July_2009'">
 +
</p>
 +
</form>
 +
</body>
 +
</html>
 +
 
 +
<html><center>
 +
<font size="6" color="#007CBC"><i>20 July 2009</i></font>
 +
</center></html>
 +
<br>
 +
----
 +
<br>
 +
<br>
 +
 
 +
 
 +
==Wet Lab==
As the transformation of Saturday didn't work, we think that the plasmid Term BBa_B0010 has a problem. We tried to begin all over with 4 other Terminator from the registery.
As the transformation of Saturday didn't work, we think that the plasmid Term BBa_B0010 has a problem. We tried to begin all over with 4 other Terminator from the registery.
Line 11: Line 37:
-
[[Team:EPF-Lausanne/Notebook/Cloning_Strategy#20.07.09|'''Cloning Strategy''']]
+
==People in the lab==
-
 
+
-
'''People in the lab'''
+
: Nathalie, Caroline, Mélanie, Tu, Heidi
: Nathalie, Caroline, Mélanie, Tu, Heidi
-
:
+
 
 +
 
 +
 
 +
<html><center><a href="https://2009.igem.org/EPF-Lausanne/18_July_2009"><img src="https://static.igem.org/mediawiki/2009/thumb/6/61/Flèche_gauche.png/70px-Flèche_gauche.png"></a> 
 +
<a href="https://2009.igem.org/EPF-Lausanne/21_July_2009"><img src="https://static.igem.org/mediawiki/2009/thumb/5/5e/Fleche_droite.png/70px-Fleche_droite.png"></a></center></html>
 +
 
 +
 
 +
</div><div CLASS="epfl09bouchon"></div>

Latest revision as of 08:58, 28 July 2009



Contents

20 July 2009




Wet Lab

As the transformation of Saturday didn't work, we think that the plasmid Term BBa_B0010 has a problem. We tried to begin all over with 4 other Terminator from the registery.

We first did an extraction of 4 new Term plasmid from kit plate. Then we did a transformation of these 4 Term + a transf. with death cassette (with the special protocole for One Shot ccdB survival cells).

At the same time, we did a PCR with the 4 plasmids from the kit + LovTAP. We purified these PCR products, in the idea to do a digestion and then a ligation with them.

In parallel, we ran a gel with the Term plasmids, but there were no bands on the gel, so we didn't do the digestion. We will wait to have the transformation products to do that.

We also prepared LB-plates : Amp-Kana, Amp-Kana-Chl and Kana-Chl.


People in the lab

Nathalie, Caroline, Mélanie, Tu, Heidi



Retrieved from "http://2009.igem.org/EPF-Lausanne/20_July_2009"