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EPF-Lausanne/7 August 2009
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==Wet Lab== | ==Wet Lab== | ||
| + | Result of the overnight incubation: Only one or two colony on 4 plates.... The positive control of the transformation grew very well. | ||
| + | Test of other ligase (Quick ligase and other T4 ligase) on LacI-RBS plasmid and GFP into two different plasmid. | ||
| + | |||
| + | Result after an overnight incubation: Nothing grew | ||
| + | |||
| + | Conclusion: We have problem with our ligation. So we need to test it without Phosphatase, with precise concentration and try a gel extraction. | ||
==People in the lab== | ==People in the lab== | ||
| - | Nath, Basile, Gab, Christian | + | Nath, Basile, Gab, Christian, Nicolas |
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Latest revision as of 09:48, 10 August 2009
Contents |
Wet Lab
Result of the overnight incubation: Only one or two colony on 4 plates.... The positive control of the transformation grew very well.
Test of other ligase (Quick ligase and other T4 ligase) on LacI-RBS plasmid and GFP into two different plasmid.
Result after an overnight incubation: Nothing grew
Conclusion: We have problem with our ligation. So we need to test it without Phosphatase, with precise concentration and try a gel extraction.
People in the lab
Nath, Basile, Gab, Christian, Nicolas
