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Uppsala-Sweden/6 August 2009
From 2009.igem.org
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Karl.brune (Talk | contribs) (New page: {{Uppsala-Sweden Template}} ==Digestion of ADH2(Z),pirB, pdc, PpetE, adh2(Y)== Digestion was performed using EcoRI and PstI from Fermentas with this protocol ([http://www.fermentas.com/f...) |
Karl.brune (Talk | contribs) (→Purification of ADH2(Z),pirB, pdc, PpetE, adh2(Y)) |
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{{Uppsala-Sweden Template}} | {{Uppsala-Sweden Template}} | ||
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| + | ==Plasmid Prep for PpetE== | ||
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| + | Plasmid prep was performed using [http://www.sigmaaldrich.com/life-science/molecular-biology/dna-and-rna-purification/plasmid-miniprep-kit.html GenElute™ Plasmid Miniprep Kit] | ||
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==Digestion of ADH2(Z),pirB, pdc, PpetE, adh2(Y)== | ==Digestion of ADH2(Z),pirB, pdc, PpetE, adh2(Y)== | ||
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==Purification of ADH2(Z),pirB, pdc, PpetE, adh2(Y)== | ==Purification of ADH2(Z),pirB, pdc, PpetE, adh2(Y)== | ||
| - | Purification of the digestion mix was performed with Nucleo Spin® Extract 2 PCR cleanup protocol from Clontech | + | Purification of the digestion mix was performed with [http://www.clontech.com/images/pt/PT3814-1.pdf Nucleo Spin® Extract 2], PCR cleanup protocol from Clontech |
Purified product (50µl) in 1.5ml eppendorfs. | Purified product (50µl) in 1.5ml eppendorfs. | ||
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--[[User:Karl.brune|Karl.brune]] 08:56, 10 August 2009 (UTC) | --[[User:Karl.brune|Karl.brune]] 08:56, 10 August 2009 (UTC) | ||
Latest revision as of 09:43, 12 August 2009
Plasmid Prep for PpetE
Plasmid prep was performed using [http://www.sigmaaldrich.com/life-science/molecular-biology/dna-and-rna-purification/plasmid-miniprep-kit.html GenElute™ Plasmid Miniprep Kit]
Digestion of ADH2(Z),pirB, pdc, PpetE, adh2(Y)
Digestion was performed using EcoRI and PstI from Fermentas with this protocol ([http://www.fermentas.com/fastdigest/index.html Protocol for Fast Digestion of Unpurified PCR Products with FastDigest® Enzymes)])
The calculated amounts for the digestion are written down here : Media:Digestion_06082009.xls
Purification of ADH2(Z),pirB, pdc, PpetE, adh2(Y)
Purification of the digestion mix was performed with [http://www.clontech.com/images/pt/PT3814-1.pdf Nucleo Spin® Extract 2], PCR cleanup protocol from Clontech
Purified product (50µl) in 1.5ml eppendorfs.
--Karl.brune 08:56, 10 August 2009 (UTC)
