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EPF-Lausanne/21 August 2009
From 2009.igem.org
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==Wet Lab== | ==Wet Lab== | ||
| + | Miniprep of the 2 possible readout 1 plasmids. | ||
| + | Colony PCR of the LacI-RBS-LovTap-Term that grew to check whether the insert is correct. Then agarose gel to verifiy length of fragment. | ||
| + | |||
| + | Also, since we wanted to do further tests on the maybe readout 1 plasmids, we did a classic PCR with the iGEM primers on the plasmids, and a digestion assay with SpeI (since the TrpOperon has 2 SpeI sites in its sequence). Checked the results on the gel as well. | ||
==People in the lab== | ==People in the lab== | ||
Revision as of 13:41, 21 August 2009
Contents |
Wet Lab
Miniprep of the 2 possible readout 1 plasmids.
Colony PCR of the LacI-RBS-LovTap-Term that grew to check whether the insert is correct. Then agarose gel to verifiy length of fragment.
Also, since we wanted to do further tests on the maybe readout 1 plasmids, we did a classic PCR with the iGEM primers on the plasmids, and a digestion assay with SpeI (since the TrpOperon has 2 SpeI sites in its sequence). Checked the results on the gel as well.
People in the lab
Basile, Gab, Christian
