EPF-Lausanne/25 August 2009

From 2009.igem.org

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: Trp operon Fwd
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{| class="wikitable" width="80%"
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|+ <big> '''Parts&Characteristics''' </big>
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|-
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! scope=col | 
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! scope=col | 2x Trp
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! scope=col | 1x Negative Control
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|-
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| width="33%" |
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[http://partsregistry.org/Part:BBa_B0010 BBa_B0010]
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| width="33%" align="center"|
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Terminator
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| width="33%" align="center" |
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A
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| width="50%" align="center" |
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13D (1)
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|-
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| width="33%" |
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[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]
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| width="33%" align="center"|
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Promoter LacI
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| width="34%" align="center" |
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A
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| width="50%" align="center" |
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1D (1)
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|-
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| width="33%" |
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[http://partsregistry.org/Part:BBa_B0030 BBa_B0030]
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| width="33%" align="center"|
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RBS
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| width="34%" align="center" |
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A
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| width="50%" align="center" |
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1H (1)
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|-
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| width="33%" |
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[http://partsregistry.org/Part:BBa_E0240 BBa_E0240]
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| width="33%" align="center"|
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RBS-GFP-TER
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| width="34%" align="center" |
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A
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| width="50%" align="center" |
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12M (1)
 +
|-
 +
| width="33%" |
 +
[http://partsregistry.org/Part:BBa_I13507 BBa_I13507]
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| width="33%" align="center"|
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RBS-mRFP-TER
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| width="34%" align="center" |
 +
A
 +
| width="50%" align="center" |
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22O (1)
 +
|-
 +
| width="33%" |
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[http://partsregistry.org/Part:BBa_J13002 BBa_J13002]
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| width="33%" align="center"|
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pTetR-RBS
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| width="34%" align="center" |
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A
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| width="50%" align="center" |
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13B (1)
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|-
 +
|}
==People in the lab==
==People in the lab==

Revision as of 11:52, 2 September 2009



Contents

25 August 2009




Wet Lab

Miniprep:

Made according to the protocol of Aisgen Miniprep Kit Elution in 50 ul. The RD2 #5 plasmids prep as some that have already been prepared will be sent for sequencing this afternoon.


Mediums

New medium were made to grow the double-transformed DH5 a (LovTap BB and RO2) -> should be without thy.

M9 was redone, pH adjusted with amino acid and filtred. for 1L:

5g glucose
6g Na2PO4
3g KH2PO4
1g NH4Cl
0.5g NaCl
0.12g MgSO4
0.01g CaCl2


Trp Operon synthesis

Using the Klenow fragments protocol and primers:£

Trp operon Rev
Trp operon Fwd
Parts&Characteristics
2x Trp 1x Negative Control

[http://partsregistry.org/Part:BBa_B0010 BBa_B0010]

Terminator

A

13D (1)

[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]

Promoter LacI

A

1D (1)

[http://partsregistry.org/Part:BBa_B0030 BBa_B0030]

RBS

A

1H (1)

[http://partsregistry.org/Part:BBa_E0240 BBa_E0240]

RBS-GFP-TER

A

12M (1)

[http://partsregistry.org/Part:BBa_I13507 BBa_I13507]

RBS-mRFP-TER

A

22O (1)

[http://partsregistry.org/Part:BBa_J13002 BBa_J13002]

pTetR-RBS

A

13B (1)

People in the lab

Nath, Basile, Gab, Christian



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