Team:Groningen/Notebook/5 September 2009
From 2009.igem.org
(Difference between revisions)
m (New page: {{Team:Groningen/Notebook/Day/Header}} ==Wet== ===GVP Cluster=== ===Transporters=== ===Metal Accumulation=== Plasmid isolation of [[Team:Groningen/Notebook/24_August_2009#Metal_Accumul...) |
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===GVP Cluster=== | ===GVP Cluster=== | ||
+ | {| border="1" | ||
+ | |+'''Michaels plates''' | ||
+ | !<u><i>E. coli</i> TOP10 Colonies</u> | ||
+ | !<u>Name</u> | ||
+ | !<u>Plasmid</u> | ||
+ | !<u>Date of plating</u> | ||
+ | !<u>Antibiotics</u><BR> | ||
+ | |- | ||
+ | |<center>92</center> | ||
+ | |pCueO-GVP conc. | ||
+ | |<partinfo>pSB2K3</partinfo> | ||
+ | |4september2009 | ||
+ | |Kanamycin/IPTG | ||
+ | |- | ||
+ | |<center>38</center> | ||
+ | |pZntR-GVP conc. | ||
+ | |<partinfo>pSB2K3</partinfo> | ||
+ | |4september2009 | ||
+ | |Kanamycin/IPTG | ||
+ | |- | ||
+ | |<center>35</center> | ||
+ | |pArsR-GVP conc. | ||
+ | |<partinfo>pSB2K3</partinfo> | ||
+ | |4september2009 | ||
+ | |Kanamycin/IPTG | ||
+ | |- | ||
+ | |<center>41</center> | ||
+ | |pLac-GVP conc. | ||
+ | |<partinfo>pSB2K3</partinfo> | ||
+ | |4september2009 | ||
+ | |Kanamycin/IPTG | ||
+ | |- | ||
+ | |<center>0</center> | ||
+ | |(7K) | ||
+ | |<partinfo>pSB2K3</partinfo> | ||
+ | |4september2009 | ||
+ | |Kanamycin/IPTG | ||
+ | |- | ||
+ | |<center>0</center> | ||
+ | |(7K) conc. | ||
+ | |<partinfo>pSB2K3</partinfo> | ||
+ | |4september2009 | ||
+ | |Kanamycin/IPTG | ||
+ | |- | ||
+ | |<center>{{todo|1}}</center> | ||
+ | |(7C) | ||
+ | |<partinfo>pSB2K3</partinfo> | ||
+ | |4september2009 | ||
+ | |Kanamycin/IPTG | ||
+ | |- | ||
+ | |<center>1</center> | ||
+ | |(7C) conc. | ||
+ | |<partinfo>pSB2K3</partinfo> | ||
+ | |4september2009 | ||
+ | |Kanamycin/IPTG | ||
+ | |- | ||
+ | |} | ||
+ | Plates with colonies were stored in fridge, the {{todo|red}} indicates a red colony. Plates without colonies were left on the bench. | ||
===Transporters=== | ===Transporters=== |
Revision as of 10:27, 5 September 2009
[http://2009.igem.org/Team:Groningen http://2009.igem.org/wiki/images/f/f1/Igemhomelogo.png]
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Wet
GVP Cluster
E. coli TOP10 Colonies | Name | Plasmid | Date of plating | Antibiotics |
---|---|---|---|---|
pCueO-GVP conc. | 4september2009 | Kanamycin/IPTG | ||
pZntR-GVP conc. | 4september2009 | Kanamycin/IPTG | ||
pArsR-GVP conc. | 4september2009 | Kanamycin/IPTG | ||
pLac-GVP conc. | 4september2009 | Kanamycin/IPTG | ||
(7K) | 4september2009 | Kanamycin/IPTG | ||
(7K) conc. | 4september2009 | Kanamycin/IPTG | ||
(7C) | 4september2009 | Kanamycin/IPTG | ||
(7C) conc. | 4september2009 | Kanamycin/IPTG |
Plates with colonies were stored in fridge, the red indicates a red colony. Plates without colonies were left on the bench.
Transporters
Metal Accumulation
Plasmid isolation of SmtA-1 ON culture
Vectors
Dry
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