Team:Groningen/Notebook/5 September 2009

From 2009.igem.org

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Wet

GVP Cluster

Michaels plates
E. coli TOP10 Colonies Name Plasmid Date of plating Antibiotics
92
pCueO-GVP conc. pSB2K3 4september2009 Kanamycin/IPTG
38
pZntR-GVP conc. pSB2K3 4september2009 Kanamycin/IPTG
35
pArsR-GVP conc. pSB2K3 4september2009 Kanamycin/IPTG
41
pLac-GVP conc. pSB2K3 4september2009 Kanamycin/IPTG
0
(7K) pSB2K3 4september2009 Kanamycin/IPTG
0
(7K) conc. pSB2K3 4september2009 Kanamycin/IPTG
1
(7C) pSB2K3 4september2009 Kanamycin/IPTG
1
(7C) conc. pSB2K3 4september2009 Kanamycin/IPTG

Plates with colonies were stored in fridge, the red indicates a red colony. Plates without colonies were left on the bench.

Transporters

Metal Accumulation

Colony PCR

Colony PCR of SmtA-1 - 05september2009.jpg

Colony PCR of SmtA colony #1 using Biobrick VF2/VR primers (lane 1) and RBSforward and SmtAreverse primers (lane 2). Probably a different RBS primer was used, explaining the lack of product. SmtA (including RBS) is 678 bp, PCR product using VF2/VR primers should yield a band of 989 bp. Left and right are both the same 1% agarose (1xTBE) gel on different time points.

Restriction analysis

Restriction analysis SmtA (pSB1AC3) with XbaI & PstI- 05september2009.jpg

Restriction analysis of of SmtA colony #1 using PstI and XbaI. Expected fragment was ~700, plasmid was pSB1AC3 about 3000 bp. 1% agarose gel (1xTBE)

Vectors

Dry

April
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
November
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30