Team:UNICAMP-Brazil/Notebooks/August 29

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=====Material=====
=====Material=====
We found a farm that creates chicken for food production, and they have donated us the organs we needed to make the RNA extraction of ''Gallus gallus'': the blood, the spleen and the oviduct.
We found a farm that creates chicken for food production, and they have donated us the organs we needed to make the RNA extraction of ''Gallus gallus'': the blood, the spleen and the oviduct.
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[[Image:chicken_run_4.jpg]]
=====RNA extraction=====
=====RNA extraction=====

Revision as of 12:23, 9 September 2009

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YeastGuard

Lysozyme

Material

We found a farm that creates chicken for food production, and they have donated us the organs we needed to make the RNA extraction of Gallus gallus: the blood, the spleen and the oviduct.

Chicken run 4.jpg

RNA extraction

We used the Trizol protocol for the three tissues (Protocol X). We obtained pellets from all tissues! Hope their concentration and quality are ok! =]

Gleidson, Raíssa, Taís and Sula

Terminator Biobrick

Today we perfomed miniprep of the Terminator Biobrick transformed E. coli, according to Protocol 2 (see protocols section).

Wesley




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