Team:UNICAMP-Brazil/Notebooks/September 14

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(New page: {{:Team:UNICAMP-Brazil/inc_topo}} {{:Team:UNICAMP-Brazil/inc calendar}} __NOTOC__ ==''' ColiGuard '''== ====finO and finP's transformed bacteria==== * All of yesterday's transformed an...)
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* All of yesterday's transformed and plated cells grew in the media!  
* All of yesterday's transformed and plated cells grew in the media!  
* We then selected 10 colonies of each transformation (finO+pSB1A3 and finP+pSB1A3) in order to confirm them. We inoculated those colonies separately into liquid LB-AMP media, and let then grow O/N at 37ºC.
* We then selected 10 colonies of each transformation (finO+pSB1A3 and finP+pSB1A3) in order to confirm them. We inoculated those colonies separately into liquid LB-AMP media, and let then grow O/N at 37ºC.
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* We will then proceed to the miniprep's of the cultures and, once we could run and agarose gel of the extracted plamids, we will be able to confirm that both finO and finP are indeed in biobrick format.
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* We will then proceed to the minipreps of the cultures and, once we could make another PCR for the inserted fragments, we will be able to confirm that both finO and finP are indeed in biobrick format.
''Marcelo''
''Marcelo''
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Revision as of 16:56, 16 September 2009

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ColiGuard

finO and finP's transformed bacteria

  • All of yesterday's transformed and plated cells grew in the media!
  • We then selected 10 colonies of each transformation (finO+pSB1A3 and finP+pSB1A3) in order to confirm them. We inoculated those colonies separately into liquid LB-AMP media, and let then grow O/N at 37ºC.
  • We will then proceed to the minipreps of the cultures and, once we could make another PCR for the inserted fragments, we will be able to confirm that both finO and finP are indeed in biobrick format.

Marcelo




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