Team:UNICAMP-Brazil/Notebooks/September 13

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==''' Yeastguard '''==
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====Lysin (orf), Jen1 (orf), DLD (promoter) and Jen1 (promoter)====
====Lysin (orf), Jen1 (orf), DLD (promoter) and Jen1 (promoter)====

Revision as of 13:25, 20 September 2009

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ColiGuard

finO+pSB1A3

  • After the O/N period, today we transformed the ligation finO+pSB1A3 into electrocompetent E. coli bacteria, strain DH10B. We followed protocol 3, without modifications (see Protocols section).
  • We then plated the transfomed cells in LB-AMP media, and let them grow at 37ºC for an O/N period.

finP+pSB1A3

  • We followed the same produce for the finP+pSB1A3 ligation.

Marcelo

YeastGuard

Lysin (orf), Jen1 (orf), DLD (promoter) and Jen1 (promoter)

  • We performed miniprep to get purified these parts (Protocol 2).
  • We then digest the plasmids obtained by the miniprep (Protocol X)

Wesley and Gleidson




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