Team:UNICAMP-Brazil/Notebooks/September 13
From 2009.igem.org
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* 6 colonies of each new biobrick were chosen of the plates made yesterday to grow in liquid LB. | * 6 colonies of each new biobrick were chosen of the plates made yesterday to grow in liquid LB. | ||
- | * Then, we performed miniprep to get our new biobricks purified | + | * Then, we performed miniprep to get our new biobricks purified [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 11]. |
* We then digested the plasmids obtained by the miniprep with ''Xba''I to confirm the correct ligation of the parts following the biobrick format (Protocolo x). However, the electrophoresis gel (shown below) showed an unexpected pattern of bands that can't confirm correctly ligations of our biobricks. =( | * We then digested the plasmids obtained by the miniprep with ''Xba''I to confirm the correct ligation of the parts following the biobrick format (Protocolo x). However, the electrophoresis gel (shown below) showed an unexpected pattern of bands that can't confirm correctly ligations of our biobricks. =( | ||
Revision as of 05:01, 3 October 2009
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