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EPF-Lausanne/21 August 2009
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Also, since we wanted to do further tests on the maybe readout 1 plasmids, we did a classic PCR with the iGEM primers on the plasmids, and a digestion assay with SpeI (since the TrpOperon has 2 SpeI sites in its sequence). Checked the results on the gel as well. | Also, since we wanted to do further tests on the maybe readout 1 plasmids, we did a classic PCR with the iGEM primers on the plasmids, and a digestion assay with SpeI (since the TrpOperon has 2 SpeI sites in its sequence). Checked the results on the gel as well. | ||
| + | |||
| + | ===Results of the first characterization=== | ||
| + | Experiment on Read Out 2. Only the clones 4, 5 and 10 had nice curves. The experiment was made using LB. For every clone there was three different possibilities : without anything OR with tryptophan OR with ATC. The cells had all exactly the same initial conditions, the products were added only at the last moment. We can see that clone 4 and 10 show a very good answer to ATC as well as to TRP. | ||
| + | |||
| + | Clone 4 : | ||
| + | [[Image:iGEM_clone4LB.jpg|center|Clone 4 plot]] | ||
| + | |||
| + | Clone 5 : | ||
| + | [[Image:iGEM_clone5LB.jpg|center|Clone 5 plot]] | ||
| + | |||
| + | Clone 10 : | ||
| + | [[Image:iGEM_clone10LB.jpg|center|Clone 10 plot]] | ||
| + | |||
==People in the lab== | ==People in the lab== | ||
Revision as of 14:37, 4 October 2009
Wet Lab
Miniprep of the 2 possible readout 1 plasmids.
Colony PCR of the LacI-RBS-LovTap-Term that grew to check whether the insert is correct. Then agarose gel to verifiy length of fragment.
Also, since we wanted to do further tests on the maybe readout 1 plasmids, we did a classic PCR with the iGEM primers on the plasmids, and a digestion assay with SpeI (since the TrpOperon has 2 SpeI sites in its sequence). Checked the results on the gel as well.
Results of the first characterization
Experiment on Read Out 2. Only the clones 4, 5 and 10 had nice curves. The experiment was made using LB. For every clone there was three different possibilities : without anything OR with tryptophan OR with ATC. The cells had all exactly the same initial conditions, the products were added only at the last moment. We can see that clone 4 and 10 show a very good answer to ATC as well as to TRP.
Clone 4 :
Clone 5 :
Clone 10 :
People in the lab
Basile, Gab, Christian
