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Team:Newcastle/Labwork/14 October 2009
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| + | =Formal Lab Session - 14th October 2009= | ||
| + | * This morning we check yesterday's transformation result, it turned out we got transformed cell for all three strains. | ||
| + | * we patched the colonies on LB+EM+CHL+Starch plate to check whether the pGFP-rrnB:kinA part were integrated in amyE part or not. | ||
| + | * After 6 hours grow on antibiotic and starch plate, we got some right colonies. | ||
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| + | [[Image:Team_Newcastle_iGEM_2009_14_10_09_1_Lab_1.jpg|300px|center]] | ||
===Microscopy Test to characterize KinA=== | ===Microscopy Test to characterize KinA=== | ||
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===Transformations=== | ===Transformations=== | ||
We did transformations from midipreps to have stock for our biobricks. We will freeze them later. We transformed ara, sac, ara+sspB Biobricks. | We did transformations from midipreps to have stock for our biobricks. We will freeze them later. We transformed ara, sac, ara+sspB Biobricks. | ||
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| + | {{:Team:Newcastle/Project/Labwork/CalTemplate}} | ||
{{:Team:Newcastle/Footer}} | {{:Team:Newcastle/Footer}} | ||
{{:Team:Newcastle/Right}} | {{:Team:Newcastle/Right}} | ||
Latest revision as of 18:13, 21 October 2009
Project
Sub-projects
Wet Lab
Meetings
Planning
Links
Formal Lab Session - 14th October 2009
- This morning we check yesterday's transformation result, it turned out we got transformed cell for all three strains.
- we patched the colonies on LB+EM+CHL+Starch plate to check whether the pGFP-rrnB:kinA part were integrated in amyE part or not.
- After 6 hours grow on antibiotic and starch plate, we got some right colonies.
Microscopy Test to characterize KinA
We inoculated 6 cultures for the microscopy test from haloless colonies since tehy were transformed with our biobrick. We inoculated them into MM competence medium overnight
- Colony 1 from BFS2426 transformation
- Colony 18 from BFS2426 transformation
- Colony 13 from BFS40 transformation
- Colony 18 from BFS240 transformation
- Colony 19 from BFS867 transformation
- Colony 22 from BFS867 transformation
- Colony 5 from BFS2426 with halo. (As a negative control)
- No cell (Control)
Transformations
We did transformations from midipreps to have stock for our biobricks. We will freeze them later. We transformed ara, sac, ara+sspB Biobricks.
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News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
Social Net
- Newcastle iGEM Twitter
- [http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
- [http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]
