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Team:UNICAMP-Brazil/Notebooks/August 17
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==''' YeastGuard '''== | ==''' YeastGuard '''== | ||
| - | ==== | + | ====Biobricks recovered: trying to purify the RBS digested fragment==== |
| - | * The RBS band purification performed on August 15 didn't work, so we digested the plasmid again. Curiously the digested band was a little bigger than we expected, so we decided to check over it's size on Registry and we found that we ressuspended the wrong biobrick!!! Fortunately it's purification didn't work before! =) | + | * The RBS band purification performed on August 15 didn't work, so we digested the plasmid again. Curiously the digested band was a little bigger than we expected , so we decided to check over it's size on Registry and we found that we ressuspended the wrong biobrick!!! Fortunately it's purification didn't work before! =) |
* After this finding we checked the other biobricks and ressuspended the correct RBS. We transformed it in ''E. coli'', plated and grew it in liquid media. | * After this finding we checked the other biobricks and ressuspended the correct RBS. We transformed it in ''E. coli'', plated and grew it in liquid media. | ||
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| + | [[Image:RBS.jpg|150px|center]] | ||
''Raíssa'' | ''Raíssa'' | ||
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==''' ColiGuard '''== | ==''' ColiGuard '''== | ||
| - | ==== | + | ====Culture for Miniprep==== |
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| + | *<p style=”text-align:justify;”>We apparently got the transformed cells; we made the cells selection and inoculated the culture in liquid medium for tomorrow's plasmid extraction.</p> | ||
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| + | ====Recovering two RBS==== | ||
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| + | *<p style=”text-align:justify;”>We recovered two biobricks:</p> | ||
| - | + | - BBa_J61100 RBS (Ribosome Binding Site Family Member) | |
| + | - BBa_J61107 RBS (Ribosome Binding Site Family Member) | ||
''Luige'' | ''Luige'' | ||
{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} | ||
Latest revision as of 01:00, 22 October 2009
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