Team:UNICAMP-Brazil/Notebooks/July 20

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* After the recovery of F plasmid (July 16th), we performed a digestion of the miniprep's product, in order to assure that we indeed recover the plasmid itself. We did this assay by using two restriction enzymes: ''EcoR''I and ''Cla''I.
* After the recovery of F plasmid (July 16th), we performed a digestion of the miniprep's product, in order to assure that we indeed recover the plasmid itself. We did this assay by using two restriction enzymes: ''EcoR''I and ''Cla''I.
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* We then compared the pattern provided us by pDRAW software with an eletrophoresis gel of the digestion product. According to this assay, we indeed were able to recover the F Plasmid!
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* We then compared the pattern provided us by pDRAW software with an electrophoresis gel of the digestion product. According to this assay, we indeed were able to recover the F Plasmid! =]
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=]
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[[image:Digestao_EcoRI_ClaI-20-07-09.jpg|center]]
[[image:Digestao_EcoRI_ClaI-20-07-09.jpg|center]]
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''Gabriel, Marcelo and Victor.''
''Gabriel, Marcelo and Victor.''
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{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Latest revision as of 00:04, 22 October 2009

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MicroGuards

Primer design

  • During the week from July 20th to 25th we designed all primers that we´re going to use in our project. All the sequences are showed on July 25th notebook.

Everybody

ColiGuard

Restriction Assay

  • After the recovery of F plasmid (July 16th), we performed a digestion of the miniprep's product, in order to assure that we indeed recover the plasmid itself. We did this assay by using two restriction enzymes: EcoRI and ClaI.
  • We then compared the pattern provided us by pDRAW software with an electrophoresis gel of the digestion product. According to this assay, we indeed were able to recover the F Plasmid! =]
Digestao EcoRI ClaI-20-07-09.jpg


Gabriel, Marcelo and Victor.





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