Team:Cambridge/Parts

From 2009.igem.org

(Difference between revisions)
(→Parts Submitted to Registry)
(→Parts Submitted to Registry)
 
(6 intermediate revisions not shown)
Line 32: Line 32:
|Reporter
|Reporter
|'''Vio operon ABCE'''. Produces a light green pigment from L-tyrosine. Formed from the vio operon biobrick (BBa_K274002) with the vioD gene removed by restriction digest with BglII and BclI. This sequence contains four genes, vioA, vioB vioC and vioE, each preceded by their own ribosome binding site.
|'''Vio operon ABCE'''. Produces a light green pigment from L-tyrosine. Formed from the vio operon biobrick (BBa_K274002) with the vioD gene removed by restriction digest with BglII and BclI. This sequence contains four genes, vioA, vioB vioC and vioE, each preceded by their own ribosome binding site.
-
| bp
+
| 6200bp
|- style="color:#333; background-color:#A3C3FF;" cellpadding="6" cellspacing="0" border="1"
|- style="color:#333; background-color:#A3C3FF;" cellpadding="6" cellspacing="0" border="1"
Line 69: Line 69:
| '''CrtEBIY under pBad promoter'''. This Biobrick is created by putting enzyme cassette CrtEBIY (with individual rbs) of Part BBa_K274200 under arabinose-induced promoter I0500. Amount of beta-carotene produced can be measured by photospectrometer with absorbance at 455nm (beta-carotene extraction using acetone).  
| '''CrtEBIY under pBad promoter'''. This Biobrick is created by putting enzyme cassette CrtEBIY (with individual rbs) of Part BBa_K274200 under arabinose-induced promoter I0500. Amount of beta-carotene produced can be measured by photospectrometer with absorbance at 455nm (beta-carotene extraction using acetone).  
| 5773bp
| 5773bp
 +
|- style="color:#333; background-color:#A3C3FF;" cellpadding="6" cellspacing="0" border="1"|
 +
 +
| <partinfo>BBa_K274111</partinfo>
 +
| Composite
 +
| '''CrtY under Pbad promoter (I0500)'''. This construct is created by putting BBa_K118013 (rbs+CrtY) under the control of Pbad promoter (I0500), by standard assembly. When used in double-transformation together with a constitutive lycopene-producing device (e.g. BBa_K274110), the resulting system can produce colour change (red to orange) upon induction with arabinose.
 +
| 2380bp
|- style="color:#333; background-color:#A3C3FF;" cellpadding="6" cellspacing="0" border="1"|  
|- style="color:#333; background-color:#A3C3FF;" cellpadding="6" cellspacing="0" border="1"|  
Line 74: Line 80:
| Composite
| Composite
| '''PoPS -> P2 ogr - PF promoter -> PoPS'''. This Biobrick is a sensitivity tuner composed of a combination of the P2 ogr phage activator and the PF phage promoter.  It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input.  
| '''PoPS -> P2 ogr - PF promoter -> PoPS'''. This Biobrick is a sensitivity tuner composed of a combination of the P2 ogr phage activator and the PF phage promoter.  It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input.  
-
| 336bp
+
| 473bp
|- style="color:#333; background-color:#A3C3FF;" cellpadding="6" cellspacing="0" border="1"|  
|- style="color:#333; background-color:#A3C3FF;" cellpadding="6" cellspacing="0" border="1"|  
 +
| <partinfo>BBa_K274371</partinfo>
 +
| Composite
 +
| '''PoPS -> P2 ogr - PO promoter -> PoPS'''. This Biobrick is a sensitivity tuner composed of a combination of the P2 ogr phage activator and the PO phage promoter.  It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input.
 +
| 474bp
 +
|- style="color:#333; background-color:#A3C3FF;" cellpadding="6" cellspacing="0" border="1"|
 +
 +
| <partinfo>BBa_K274374</partinfo>
 +
| Composite
 +
| '''PoPS -> P2 ogr - Psid promoter -> PoPS'''. This Biobrick is a sensitivity tuner composed of a combination of the P2 ogr phage activator and the Psid phage promoter.  It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input.
 +
| 475bp
 +
|- style="color:#333; background-color:#A3C3FF;" cellpadding="6" cellspacing="0" border="1"|
 +
 +
| <partinfo>BBa_K274375</partinfo>
 +
| Composite
 +
| '''PoPS -> P2 ogr - PLL promoter -> PoPS'''. This Biobrick is a sensitivity tuner composed of a combination of the P2 ogr phage activator and the PLL phage promoter.  It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input.
 +
| 474bp
 +
|- style="color:#333; background-color:#A3C3FF;" cellpadding="6" cellspacing="0" border="1"|
 +
 +
| <partinfo>BBa_K274380</partinfo>
 +
| Composite
 +
| '''PoPS -> PSP3 pag - PF promoter -> PoPS'''. This Biobrick is a sensitivity tuner composed of a combination of the PSP3 pag phage activator and the PFF phage promoter.  It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input.
 +
| 473bp
 +
|- style="color:#333; background-color:#A3C3FF;" cellpadding="6" cellspacing="0" border="1"|
 +
 +
| <partinfo>BBa_K274381</partinfo>
 +
| Composite
 +
| '''PoPS -> PSP3 pag - PO promoter -> PoPS'''. This Biobrick is a sensitivity tuner composed of a combination of the PSP3 pag phage activator and the PO phage promoter.  It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input.
 +
| 474bp
 +
|- style="color:#333; background-color:#A3C3FF;" cellpadding="6" cellspacing="0" border="1"|
 +
 +
| <partinfo>BBa_K274382</partinfo>
 +
| Composite
 +
| '''PoPS -> PSP3 pag - PP promoter -> PoPS'''. This Biobrick is a sensitivity tuner composed of a combination of the PSP3 pag phage activator and the PP phage promoter.  It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input.
 +
| 474bp
 +
|- style="color:#333; background-color:#A3C3FF;" cellpadding="6" cellspacing="0" border="1"|
 +
 +
| <partinfo>BBa_K274384</partinfo>
 +
| Composite
 +
| '''PoPS -> PSP3 pag - Psid promoter -> PoPS'''. This Biobrick is a sensitivity tuner composed of a combination of the PSP3 pag phage activator and the Psid phage promoter.  It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input.
 +
| 475bp
 +
|- style="color:#333; background-color:#A3C3FF;" cellpadding="6" cellspacing="0" border="1"|
 +
 +
| <partinfo>BBa_K274391</partinfo>
 +
| Composite
 +
| '''PoPS -> PhiR73 pag - PO promoter -> PoPS'''. This Biobrick is a sensitivity tuner composed of a combination of the PhiR73 pag phage activator and the PO phage promoter.  It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input.
 +
| 501bp
 +
|- style="color:#333; background-color:#A3C3FF;" cellpadding="6" cellspacing="0" border="1"|
 +
 +
| <partinfo>BBa_K274392</partinfo>
 +
| Composite
 +
| '''PoPS -> PhiR73 pag - PP promoter -> PoPS'''. This Biobrick is a sensitivity tuner composed of a combination of the PhiR73 pag phage activator and the PP phage promoter.  It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input.
 +
| 501bp
 +
|- style="color:#333; background-color:#A3C3FF;" cellpadding="6" cellspacing="0" border="1"|
 +
 +
| <partinfo>BBa_K274394</partinfo>
 +
| Composite
 +
| '''PoPS -> PhiR73 pag - Psid promoter -> PoPS'''. This Biobrick is a sensitivity tuner composed of a combination of the PhiR73 pag phage activator and the Psid phage promoter.  It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input.
 +
| 502bp
 +
|- style="color:#333; background-color:#A3C3FF;" cellpadding="6" cellspacing="0" border="1"|
 +
 +
| <partinfo>BBa_K274395</partinfo>
 +
| Composite
 +
| '''PoPS -> PhiR73 pag - PLL promoter -> PoPS'''. This Biobrick is a sensitivity tuner composed of a combination of the PhiR73 pag phage activator and the PLL phage promoter.  It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input.
 +
| 501bp
 +
|- style="color:#333; background-color:#A3C3FF;" cellpadding="6" cellspacing="0" border="1"|
|}
|}
 +
 +
 +

Latest revision as of 12:51, 21 October 2009


Parts Submitted to Registry

Registry Code Type Sequence Description / Notes Length
Reporter MelA. The gene (melA) codes for a tyrosinase which produces a dark brown pigment from L-tyrosine. Production of the pigment requires the addition of copper and L-tyrosine supplements (the copper acts as a cofactor for the gene product) but no other precursors. The BioBrick sequence includes the native ribosome binding site. 1844bp
Reporter Violacein. Produces a purple pigment (violacein) from L-tyrosine. The operon contains five genes (VioA-E) each with their own ribosome binding sites. 7346bp
Reporter Vio operon ABDE. Produces a dark green pigment from L-tyrosine. Formed from the vio operon biobrick (BBa_K274002) with the vioC gene removed by restriction digest with BamHI and BglII. This sequence contains four genes, vioA, vioB vioD and vioE, each preceded by their own ribosome binding site. 6032bp
Reporter Vio operon ABCE. Produces a light green pigment from L-tyrosine. Formed from the vio operon biobrick (BBa_K274002) with the vioD gene removed by restriction digest with BglII and BclI. This sequence contains four genes, vioA, vioB vioC and vioE, each preceded by their own ribosome binding site. 6200bp
Composite CrtEBI with rbs. This Composite Biobrick is created by standard assembly of 3 basic Biobricks coding for enzymes CrtE, CrtB and CrtI (each with rbs). Together, enzymes CrtE, CrtB and CrtI convert colourless farnesyl pyrophosphate to red lycopene (via intermediates geranylgeranyl pyroiphosphate and phytoene). The red lycopene pigment can then be used as a coloured signal output, e.g. for biosensors. 3385bp
Generator CrtEBI under constitutive promoter. This Biobrick is created by putting enzyme cassette CrtEBI (with individual rbs) of Part BBa_K274100 under constitutive promoter R0011. Amount of lycopene produced can be measured by photospectrometer with absorbance at 475nm (lycopene extraction using acetone). 3448bp
Generator CrtEBI under pBad promoter. This Biobrick is created by putting enzyme cassette CrtEBI (with individual rbs) of Part BBa_K274100 under arabinose-induced promoter I0500. Amount of lycopene produced can be measured by photospectrometer with absorbance at 475nm (lycopene extraction using acetone). 4603bp
Composite CrtEBIY with rbs. This Composite Biobrick is created by standard assembly of 4 basic Biobricks coding for enzymes CrtE, CrtB, CrtI and CrtY (each with rbs). Together, enzymes CrtE, CrtB, CrtI and CrtY convert colourless farnesyl pyrophosphate to orange beta-carotene (via intermediates geranylgeranyl pyroiphosphate, phytoene and lycopene). The orange beta-carotene pigment can then be used as a coloured signal output, e.g. for biosensors. 4555bp
Generator CrtEBIY under constitutive promoter. This Biobrick is created by putting enzyme cassette CrtEBIY (with individual rbs) of Part BBa_K274200 under constitutive promoter R0011. Amount of beta-carotene produced can be measured by photospectrometer with absorbance at 455nm (beta-carotene extraction using acetone). 4618bp
Generator CrtEBIY under pBad promoter. This Biobrick is created by putting enzyme cassette CrtEBIY (with individual rbs) of Part BBa_K274200 under arabinose-induced promoter I0500. Amount of beta-carotene produced can be measured by photospectrometer with absorbance at 455nm (beta-carotene extraction using acetone). 5773bp
Composite CrtY under Pbad promoter (I0500). This construct is created by putting BBa_K118013 (rbs+CrtY) under the control of Pbad promoter (I0500), by standard assembly. When used in double-transformation together with a constitutive lycopene-producing device (e.g. BBa_K274110), the resulting system can produce colour change (red to orange) upon induction with arabinose. 2380bp
Composite PoPS -> P2 ogr - PF promoter -> PoPS. This Biobrick is a sensitivity tuner composed of a combination of the P2 ogr phage activator and the PF phage promoter. It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input. 473bp
Composite PoPS -> P2 ogr - PO promoter -> PoPS. This Biobrick is a sensitivity tuner composed of a combination of the P2 ogr phage activator and the PO phage promoter. It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input. 474bp
Composite PoPS -> P2 ogr - Psid promoter -> PoPS. This Biobrick is a sensitivity tuner composed of a combination of the P2 ogr phage activator and the Psid phage promoter. It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input. 475bp
Composite PoPS -> P2 ogr - PLL promoter -> PoPS. This Biobrick is a sensitivity tuner composed of a combination of the P2 ogr phage activator and the PLL phage promoter. It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input. 474bp
Composite PoPS -> PSP3 pag - PF promoter -> PoPS. This Biobrick is a sensitivity tuner composed of a combination of the PSP3 pag phage activator and the PFF phage promoter. It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input. 473bp
Composite PoPS -> PSP3 pag - PO promoter -> PoPS. This Biobrick is a sensitivity tuner composed of a combination of the PSP3 pag phage activator and the PO phage promoter. It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input. 474bp
Composite PoPS -> PSP3 pag - PP promoter -> PoPS. This Biobrick is a sensitivity tuner composed of a combination of the PSP3 pag phage activator and the PP phage promoter. It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input. 474bp
Composite PoPS -> PSP3 pag - Psid promoter -> PoPS. This Biobrick is a sensitivity tuner composed of a combination of the PSP3 pag phage activator and the Psid phage promoter. It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input. 475bp
Composite PoPS -> PhiR73 pag - PO promoter -> PoPS. This Biobrick is a sensitivity tuner composed of a combination of the PhiR73 pag phage activator and the PO phage promoter. It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input. 501bp
Composite PoPS -> PhiR73 pag - PP promoter -> PoPS. This Biobrick is a sensitivity tuner composed of a combination of the PhiR73 pag phage activator and the PP phage promoter. It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input. 501bp
Composite PoPS -> PhiR73 pag - Psid promoter -> PoPS. This Biobrick is a sensitivity tuner composed of a combination of the PhiR73 pag phage activator and the Psid phage promoter. It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input. 502bp
Composite PoPS -> PhiR73 pag - PLL promoter -> PoPS. This Biobrick is a sensitivity tuner composed of a combination of the PhiR73 pag phage activator and the PLL phage promoter. It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input. 501bp




Cambridge Sponsor Logo1.pngCambridge Sponsor Logo2.pngCambridge Sponsor Logo3.pngCambridge Sponsor Logo4.pngCambridge Sponsor Logo5.pngCambridge Sponsor Logo8.pngCambridge Sponsor Logo6.pngCambridge Sponsor Logo7.pngCambridge Sponsor Logo9.pngCambridge Sponsor Logo10.pngCambridge Sponsor Logo11.pngCambridge Sponsor Logo12.pngCambridge Sponsor Logo14.pngCambridge Sponsor Logo13.pngCambridge Sponsor Logo15.pngCambridge Sponsor Logo16.pngCambridge Sponsor Logo17.pngCambridge Sponsor Logo18.pngCambridge Sponsor Logo19.pngBmglab.jpg

Retrieved from "http://2009.igem.org/Team:Cambridge/Parts"