Team:Brown/Notebook Meetings/5-4-09

From 2009.igem.org

(Difference between revisions)

Latest revision as of 02:13, 22 October 2009

iGEM 2009 Meeting

May 4, 2009

6:13PM: Meeting starts in SFH Auditorium. Discussed War War Ko’s name.
6:14: Project ideas. Adding Minoo’s “Pro Bread” to the list
- Yeast that makes amino acids (I, L, V) and packages
6:25: Pulling out E. Colirs
6:29: Stephanie suggests T shirts
6:31: https://2009.igem.org/Team:Brown
- This is the iGEM Team Wiki that we can customize
6:37: Tick and Treat
- Need to find out what histamine does after it binds to a histamine receptor
- EV131 HBP is a potential part to submit
- Addressed the liver targeting protein issue – need to control this to make sure it grabs a histamine before it is uptaked by liver
- Delivery system
- Timeline?
7:01: Safe Cell
- Will – “This is how we can prevent government regulation”
- Implementation: RFC biobrick – as part of the standard for making a device, you would have to have these genes included.
- Applicable to prokaryotes and eukayotes
- Media vs gel – we can put the repressor in the gel
- Refine holin slide
- Transcriptional repressor implementation?
- Indu will make it asthetically pleasing

7:21: Pro Bread
- Background is good but we should get to the point quicker
- Issues: do yeast have all the enzymes to make the essential amino acids?
- Yes. If so, then we can upregulate these enzymes
- Can we produce gram quantities?
- Will depleting pyruvate affect cells ability to make amino acids because we are depleting some of its ATP source
- Can we FIND the rate limiting step of yeast ILV biosynthesis pathways? Upregulating this step will produce more amino acids
- Negative feedback – can we prevent this? Packaging?

7:54: Eli’s Cog Sci Project
7:58: Histamine: Indu, Eli, Ashley
- Safe Cell: Ahmad, Michael, Flora
- Pro Bread: Minoo, Will, Steph

7:59: NEXT MEETING: Thursday afternoon 3:00 PM
- BRING YOUR FINAL POWERPOINTS!! And make the changes.

@@ Line 8: / Line 8: @@
-iGEM 2009 Meeting
+'''iGEM 2009 Meeting'''
-May 4, 2009
-:13PM: Meeting starts in SFH Auditorium. Discussed War War Ko’s name.
+'''May 4, 2009'''
-:14: Project ideas. Adding Minoo’s “Pro Bread” to the list
-	Yeast that makes amino acids (I, L, V) and packages
+*6:13PM: Meeting starts in SFH Auditorium. Discussed War War Ko’s name.
-:25: Pulling out E. Colirs
+*6:14: Project ideas. Adding Minoo’s “Pro Bread” to the list
-:29: Stephanie suggests T shirts
+**Yeast that makes amino acids (I, L, V) and packages
-:31: https://2009.igem.org/Team:Brown
+*6:25: Pulling out E. Colirs
-	This is the iGEM Team Wiki that we can customize
+*6:29: Stephanie suggests T shirts
-:37: Tick and Treat
+*6:31: https://2009.igem.org/Team:Brown
-	Need to find out what histamine does after it binds to a histamine receptor
+**This is the iGEM Team Wiki that we can customize
-	EV131 HBP is a potential part to submit
+*6:37: Tick and Treat
-	Addressed the liver targeting protein issue – need to control this to make sure it grabs a histamine before it is uptaked by liver
+**Need to find out what histamine does after it binds to a histamine receptor
-	Delivery system
+**EV131 HBP is a potential part to submit
-	Timeline?
+**Addressed the liver targeting protein issue – need to control this to make sure it grabs a histamine before it is uptaked by liver
-:01: Safe Cell
+**Delivery system
-	Will – “This is how we can prevent government regulation”
+**Timeline?
-	Implementation: RFC biobrick – as part of the standard for making a device, you would have to have these genes included.
+*7:01: Safe Cell
-	Applicable to prokaryotes and eukayotes
+**Will – “This is how we can prevent government regulation”
-	Media vs gel – we can put the repressor in the gel
+**Implementation: RFC biobrick – as part of the standard for making a device, you would have to have these genes included.
-	Refine holin slide
+**Applicable to prokaryotes and eukayotes
-	Transcriptional repressor implementation?
+**Media vs gel – we can put the repressor in the gel
-	Indu will make it asthetically pleasing
+**Refine holin slide
-:21: Pro Bread
+**Transcriptional repressor implementation?
-	Background is good but we should get to the point quicker
+**Indu will make it asthetically pleasing
-	Issues: do yeast have all the enzymes to make the essential amino acids?
-	Yes. If so, then we can upregulate these enzymes
+*7:21: Pro Bread
-	Can we produce gram quantities?
+**Background is good but we should get to the point quicker
-	Will depleting pyruvate affect cells ability to make amino acids because we are depleting some of its ATP source
+**Issues: do yeast have all the enzymes to make the essential amino acids?
-	Can we FIND the rate limiting step of yeast ILV biosynthesis pathways? Upregulating this step will produce more amino acids
+**Yes. If so, then we can upregulate these enzymes
-	Negative feedback – can we prevent this? Packaging?
+**Can we produce gram quantities?
-:54: Eli’s Cog Sci Project
+**Will depleting pyruvate affect cells ability to make amino acids because we are depleting some of its ATP source
-:58: Histamine: Indu, Eli, Ashley
+**Can we FIND the rate limiting step of yeast ILV biosynthesis pathways? Upregulating this step will produce more amino acids
-	Safe Cell: Ahmad, Michael, Flora
+**Negative feedback – can we prevent this? Packaging?
-	Pro Bread: Minoo, Will, Steph
-:59: NEXT MEETING: Thursday afternoon 3:00 PM
+*7:54: Eli’s Cog Sci Project
-	BRING YOUR FINAL POWERPOINTS!! And make the changes.
+*7:58: Histamine: Indu, Eli, Ashley
+**Safe Cell: Ahmad, Michael, Flora
+**Pro Bread: Minoo, Will, Steph
+*7:59: NEXT MEETING: Thursday afternoon 3:00 PM
+**BRING YOUR FINAL POWERPOINTS!! And make the changes.