Team:UNICAMP-Brazil/Notebooks/September 28
From 2009.igem.org
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==== CeaB and CeiB transformation into plasmid [http://partsregistry.org/Part:pSB1A3 pSBA3] ==== | ==== CeaB and CeiB transformation into plasmid [http://partsregistry.org/Part:pSB1A3 pSBA3] ==== | ||
- | *<p style=”text-align:justify;”>Today, we dephosphorilated the plasmid [http://partsregistry.org/Part:pSB1A3 pSBA3], immediately started the ligation reaction of this unphosphorylated plasmid with CeaB and CeiB | + | *<p style=”text-align:justify;”>Today, we dephosphorilated the plasmid [http://partsregistry.org/Part:pSB1A3 pSBA3], and immediately started the ligation reaction of this unphosphorylated plasmid with CeaB and CeiB. Considering that the dephosphorylation use buffer that contains salt and that salt could be interfering in the transformations, we performed a dyalisis to get the samples free of salts. At last, we transformed competent ''E. coli'' (according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Electroporation protocol 3]) and plated the respective plates. This time we expect to obtain transforming cells.</p> |
''Luige'' | ''Luige'' | ||
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==''' YeastGuard '''== | ==''' YeastGuard '''== |
Latest revision as of 03:26, 22 October 2009
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