Team:KU Seoul/Overview
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== Overview == | == Overview == | ||
- | + | ::'''Integrated Heavy Metal Detection System''' | |
- | [[Image: | + | :::Our team project is designing synthetic modules for simultaneous detection of multiple heavy metals such as |
- | [[Image: | + | :arsenic, zinc, and cadmium in E. coli. The ultimate goal is to build a micromachine sensing and determining of |
- | [[Image: | + | :the concentration of heavy metals in a sample solution (e.g. the waste water). In order to design the system, |
- | [[Image: | + | :we will employ two fluorescence proteins (GFP and RFP) and aryl acylamidase as signal reporters. Since each |
+ | :heavy metal promoter produces unique fluorescence or color by those reporters, if more than two heavy metals | ||
+ | :coexist in a solution, the results would be interpreted from the convoluted fluorescence and/or color rather | ||
+ | :than a single signal detection. The successful construction of the synthetic modules in E. coli can be utilized | ||
+ | :in the form of a lyophilized powder, which can be stored in a drug capsule to make it portable. | ||
+ | |||
+ | ::'''STEP1, Prepare plasmids.''' | ||
+ | [[Image:KU_plasmids.png|center]] | ||
+ | :: | ||
+ | :: | ||
+ | :: | ||
+ | :: | ||
+ | :: | ||
+ | ::'''STEP2. Gene cloning & Infusion Biobrick assembly(SLIC)''' | ||
+ | :Using the SLIC method, infuse the biobricks inside of plasmid. There are two inserts just made through SLIC method. | ||
+ | ::::[[Image:insert_yodA_amd.png|center]] | ||
+ | :::: | ||
+ | [[Image:Insert_ars_znt.png|center]] | ||
+ | :: | ||
+ | ::'''STEP3.measuring the concentration of heavy metal''' | ||
+ | |||
+ | ::[[Image:cd2+_detector.png|center]] | ||
+ | :This picture is showing cadimum detection. Another case is similar to this experiments. | ||
+ | |||
|} | |} |
Latest revision as of 14:12, 21 October 2009
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Overview
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