Team:UNICAMP-Brazil/Notebooks/August 21

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==''' YeastGuard '''==
==''' YeastGuard '''==
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==''' YeastGuard '''==
 
'''Still fighting for RBS'''
'''Still fighting for RBS'''
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* We digested more plasmids with the ''Spe''I and ''Pst''I enzymes([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Digestion_reaction Protocol 14]). We left it digest a little longer and put less amount of DNA into the reaction. This time it seamed to work.
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* We digested more plasmids with the ''Spe''I and ''Pst''I enzymes ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Digestion_reaction Protocol 14]). We left it digesting a little longer and put less amount of DNA into the reaction. This time it seamed to work.
[[image:20090822_Rá_digestao 2 rbs 22_08_09.JPG|150px|center]]   
[[image:20090822_Rá_digestao 2 rbs 22_08_09.JPG|150px|center]]   

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YeastGuard

Still fighting for RBS

  • We digested more plasmids with the SpeI and PstI enzymes (Protocol 14). We left it digesting a little longer and put less amount of DNA into the reaction. This time it seamed to work.
20090822 Rá digestao 2 rbs 22 08 09.JPG

Raíssa




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