"
Deposited BioBricks.html
From 2009.igem.org
(Difference between revisions)
| (5 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| - | {| | + | {{SLOtemp| |
| - | + | Content= | |
| - | + | __NOTOC__ | |
| - | + | <br> | |
| - | + | <html><center> | |
| - | + | <font size="6" color="#009ee0"><b>Deposited BioBricks</b></font> | |
| - | + | </center></html> | |
| - | + | <br> | |
| - | + | <br> | |
| - | + | ==Designed BioBricks== | |
| - | + | We have designed four NEW functionalised linker-extension standard vectors. All together, 106 parts were generated all cloned into our NEW functionalised linker-extension standard vectors and forty-one were single coding, thirteen intermediates and forty-eight composite parts. | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ==Plasmid backbones== | |
| - | + | Four NEW linker-extension standard vectors are constructed. | |
| - | + | ||
| - | + | <br> | |
| - | + | ||
| - | + | <table class="crte"> | |
| - | + | <tr><td><b>Name</b></td><td><b>Type</b></td><td><b>Description</b></td><td><b>Length</b></td></tr> | |
| - | + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245005 BBa_K245005]</td><td>Plasmid_Backbone</td><td>BB-NIC-II-HisN</td><td>2261</td></tr> | |
| - | + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245006 BBa_K245006]</td><td>Plasmid_Backbone</td><td>BB-NIC-II-NC</td><td>2240</td></tr> | |
| - | + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245007 BBa_K245007]</td><td>Plasmid_Backbone</td><td>BB-NIC-II-HisC</td><td>2167</td></tr> | |
| - | + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245008 BBa_K245008]</td><td>Plasmid_Backbone</td><td>BB-NIC-III-HisN</td><td>2261</td></tr> | |
| - | + | </table> | |
| - | + | ||
| - | + | <br> | |
| - | + | ||
| - | + | ==The coding sequences== | |
| - | + | The forty-one BioBrick coding sequences are prepared and are listed as (i) coiled-coil-forming segments, (ii) oligimerisation domains, (iii) functional domains and others. | |
| - | + | <br> | |
| - | + | ===Coiled-coil segments=== | |
| - | + | <table class="crte"> | |
| - | + | <tr><td><b>Name</b></td><td><b>Type</b></td><td><b>Description</b></td><td><b>Length</b></td></tr> | |
| - | + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245038 BBa_K245038]</td><td>Coding</td><td>NZ - cc homodimer</td><td>87</td></tr> | |
| - | + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245039 BBa_K245039]</td><td>Coding</td><td>CZ</td><td>90</td></tr> | |
| - | + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245118 BBa_K245118]</td><td>Coding</td><td>P1</td><td>99</td></tr> | |
| - | + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245119 BBa_K245119]</td><td>Coding</td><td>P2</td><td>99</td></tr> | |
| - | + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245120 BBa_K245120]</td><td>Coding</td><td>P3</td><td>99</td></tr> | |
| - | + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245121 BBa_K245121]</td><td>Coding</td><td>P4</td><td>99</td></tr> | |
| - | + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245122 BBa_K245122]</td><td>Coding</td><td>P5</td><td>99</td></tr> | |
| - | + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245123 BBa_K245123]</td><td>Coding</td><td>P6</td><td>99</td></tr> | |
| - | + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245124 BBa_K245124]</td><td>Coding</td><td>P7</td><td>99</td></tr> | |
| - | + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245125 BBa_K245125]</td><td>Coding</td><td>P8</td><td>99</td></tr> | |
| - | + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245126 BBa_K245126]</td><td>Coding</td><td>H1</td><td>99</td></tr> | |
| - | + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245127 BBa_K245127]</td><td>Coding</td><td>GCN</td><td>99</td></tr> | |
| - | + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245132 BBa_K245132]</td><td>Coding</td><td>APH1</td><td>90</td></tr> | |
| - | + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245133 BBa_K245133]</td><td>Coding</td><td>APH</td><td>135</td></tr> | |
| - | + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245134 BBa_K245134]</td><td>Coding</td><td>BCR</td><td>108</td></tr> | |
| + | </table> | ||
| + | |||
| + | <br> | ||
| + | ===Oligomerization domains=== | ||
| + | <table class="crte"> | ||
| + | <tr><td><b>Name</b></td><td><b>Type</b></td><td><b>Description</b></td><td><b>Length</b></td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245026 BBa_K245026]</td><td>Coding</td><td>TA</td><td>63</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245027 BBa_K245027]</td><td>Coding</td><td>TB</td><td>63</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245028 BBa_K245028]</td><td>Coding</td><td>TC</td><td>63</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245029 BBa_K245029]</td><td>Coding</td><td>T1W_DDD</td><td>189</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245030 BBa_K245030]</td><td>Coding</td><td>T1W_KKK</td><td>189</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245031 BBa_K245031]</td><td>Coding</td><td>T1W_EEE</td><td>186</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245042 BBa_K245042]</td><td>Coding</td><td>VASP</td><td>135</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245116 BBa_K245116]</td><td>Coding</td><td>GyrB</td><td>657</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245117 BBa_K245117]</td><td>Coding</td><td>GyrB-SG</td><td>663</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245128 BBa_K245128]</td><td>Coding</td><td>p53</td><td>90</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245129 BBa_K245129]</td><td>Coding</td><td>CutA1</td><td>333</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245130 BBa_K245130]</td><td>Coding</td><td>T1W_AEA</td><td>186</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245141 BBa_K245141]</td><td>Coding</td><td>foldon</td><td>81</td></tr> | ||
| + | </table> | ||
| + | |||
| + | <br> | ||
| + | ===Functional domains=== | ||
| + | <table class="crte"> | ||
| + | <tr><td><b>Name</b></td><td><b>Type</b></td><td><b>Description</b></td><td><b>Length</b></td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245021 BBa_K245021]</td><td>Coding</td><td>cYFP</td><td>252</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245022 BBa_K245022]</td><td>Coding</td><td>nYFP</td><td>462</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245024 BBa_K245024]</td><td>Coding</td><td>Z1</td><td>36</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245025 BBa_K245025]</td><td>Coding</td><td>AUP</td><td>36</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245046 BBa_K245046]</td><td>Coding</td><td>ccdB</td><td>306</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245113 BBa_K245113]</td><td>Coding</td><td>KSI-DP</td><td>378</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245114 BBa_K245114]</td><td>Coding</td><td>LL37</td><td>111</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245115 BBa_K245115]</td><td>Coding</td><td>KSI-DP-P2/33</td><td>405</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245140 BBa_K245140]</td><td>Coding</td><td>mNGF</td><td>669</td></tr> | ||
| + | </table> | ||
| + | |||
| + | <br> | ||
| + | ===Others=== | ||
| + | <table class="crte"> | ||
| + | <tr><td><b>Name</b></td><td><b>Type</b></td><td><b>Description</b></td><td><b>Length</b></td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245131 BBa_K245131]</td><td>Coding</td><td>ELST</td><td>150</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245139 BBa_K245139]</td><td>Coding</td><td>RADA</td><td>66</td></tr> | ||
| + | </table> | ||
| + | |||
| + | <br> | ||
| + | <br> | ||
| + | ==The intermediate sequences== | ||
| + | Thirteen BioBricks with intermediate parts are constructed. | ||
| + | <br> | ||
| + | <table class="crte"> | ||
| + | <tr><td><b>Name</b></td><td><b>Type</b></td><td><b>Description</b></td><td><b>Length</b></td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245048 BBa_K245048]</td><td>Intermediate</td><td>H1-P2</td><td>204</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245049 BBa_K245049]</td><td>Intermediate</td><td>KSI-DP-P1</td><td>489</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245052 BBa_K245052]</td><td>Intermediate</td><td>GCN_P2</td><td>204</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245053 BBa_K245053]</td><td>Intermediate</td><td>P4-H1</td><td>204</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245054 BBa_K245054]</td><td>Intermediate</td><td>P2-H1</td><td>204</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245055 BBa_K245055]</td><td>Intermediate</td><td>P6-H1</td><td>204</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245056 BBa_K245056]</td><td>Intermediate</td><td>P3-P5</td><td>204</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245057 BBa_K245057]</td><td>Intermediate</td><td>P4-P2</td><td>204</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245059 BBa_K245059]</td><td>Intermediate</td><td>LL37-ELST</td><td>267</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245060 BBa_K245060]</td><td>Intermediate</td><td>ELST-ELST-ELST</td><td>462</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245061 BBa_K245061]</td><td>Intermediate</td><td>ELST-ELST</td><td>306</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245096 BBa_K245096]</td><td>Intermediate</td><td>BCR-P2</td><td>213</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245097 BBa_K245097]</td><td>Intermediate</td><td>APH-P2</td><td>240</td></tr> | ||
| + | </table> | ||
| + | |||
| + | <br> | ||
| + | <br> | ||
| + | |||
| + | ==Composite parts== | ||
| + | The forty-eight BioBricks as composites are prepared, which are used in (i) polypeptide manufacturing, (ii) polypeptide membranes, (iii) coiled-coil polyhedra, (iv) regulated assembly and (v) functional biopolymers. | ||
| + | |||
| + | <br> | ||
| + | ===Polypeptide manufacturing=== | ||
| + | <table class="crte"> | ||
| + | <tr><td><b>Name</b></td><td><b>Type</b></td><td><b>Description</b></td><td><b>Length</b></td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245115 BBa_K245115]</td><td>Coding</td><td>KSI-DP-P2/33</td><td>405</td></tr> | ||
| + | </table> | ||
| + | |||
| + | <br> | ||
| + | ===Polypeptide membrane=== | ||
| + | <table class="crte"> | ||
| + | <tr><td><b>Name</b></td><td><b>Type</b></td><td><b>Description</b></td><td><b>Length</b></td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245091 BBa_K245091]</td><td>Composite</td><td>p53-APH1</td><td>186</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245092 BBa_K245092]</td><td>Composite</td><td>p53-APH</td><td>231</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245093 BBa_K245093]</td><td>Composite</td><td>p53-BCR</td><td>204</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245099 BBa_K245099]</td><td>Composite</td><td>APH-p53</td><td>231</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245100 BBa_K245100]</td><td>Composite</td><td>BCR-p53</td><td>204</td></tr> | ||
| + | </table> | ||
| + | |||
| + | <br> | ||
| + | ===Coiled-coil polyhedra=== | ||
| + | <table class="crte"> | ||
| + | <tr><td><b>Name</b></td><td><b>Type</b></td><td><b>Description</b></td><td><b>Length</b></td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245066 BBa_K245066]</td><td>Composite</td><td>P1-GCN-P2</td><td>309</td></tr> | ||
| + | </table> | ||
| + | |||
| + | <br> | ||
| + | ===Regulated assembly=== | ||
| + | <table class="crte"> | ||
| + | <tr><td><b>Name</b></td><td><b>Type</b></td><td><b>Description</b></td><td><b>Length</b></td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245098 BBa_K245098]</td><td>Composite</td><td>GyrB-foldon</td><td>744</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245050 BBa_K245050]</td><td>Composite</td><td>GyrB-CutA1</td><td>996</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245051 BBa_K245051]</td><td>Composite</td><td>GyrB-SG-SG-CutA1</td><td>1002</td></tr> | ||
| + | </table> | ||
| + | |||
| + | <br> | ||
| + | ===Functional biopolymers=== | ||
| + | <table class="crte"> | ||
| + | <tr><td><b>Name</b></td><td><b>Type</b></td><td><b>Description</b></td><td><b>Length</b></td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245102 BBa_K245102]</td><td>Composite</td><td>LL37-ELST-GCN-ELST-P2</td><td>633</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245105 BBa_K245105]</td><td>Composite</td><td>ELST-ELST-ELST-mNGF</td><td>1137</td></tr> | ||
| + | </table> | ||
| + | |||
| + | <br> | ||
| + | <br> | ||
| + | =Slovenia 2009 iGEM Team Parts Sandbox= | ||
| + | <br> | ||
| + | <br> | ||
| + | <table class="crte"> | ||
| + | <tr><td><b>Name</b></td><td><b>Type</b></td><td><b>Description</b></td><td><b>Length</b></td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245005 BBa_K245005]</td><td>Plasmid_Backbone</td><td>BB-NIC-II-HisN</td><td>2261</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245006 BBa_K245006]</td><td>Plasmid_Backbone</td><td>BB-NIC-II-NC</td><td>2240</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245007 BBa_K245007]</td><td>Plasmid_Backbone</td><td>BB-NIC-II-HisC</td><td>2167</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245008 BBa_K245008]</td><td>Plasmid_Backbone</td><td>BB-NIC-III-HisN</td><td>2261</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245009 BBa_K245009]</td><td>DNA</td><td><nowiki>RFC</nowiki> 37 Scar Sequence</td><td>6</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245021 BBa_K245021]</td><td>Coding</td><td>cYFP</td><td>252</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245022 BBa_K245022]</td><td>Coding</td><td>nYFP</td><td>462 </td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245024 BBa_K245024]</td><td>Coding</td><td>Z1</td><td>36</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245025 BBa_K245025]</td><td>Coding</td><td>AUP</td><td>36</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245026 BBa_K245026]</td><td>Coding</td><td>TA</td><td>63</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245027 BBa_K245027]</td><td>Coding</td><td>TB</td><td>63</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245028 BBa_K245028]</td><td>Coding</td><td>TC</td><td>63</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245029 BBa_K245029]</td><td>Coding</td><td>T1W_DDD</td><td>189</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245030 BBa_K245030]</td><td>Coding</td><td>T1W_KKK</td><td>189</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245031 BBa_K245031]</td><td>Coding</td><td>T1W_EEE</td><td>186</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245038 BBa_K245038]</td><td>Coding</td><td>NZ - cc homodimer</td><td>87</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245039 BBa_K245039]</td><td>Coding</td><td>CZ</td><td>90</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245042 BBa_K245042]</td><td>Coding</td><td>VASP</td><td>135</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245046 BBa_K245046]</td><td>Coding</td><td>ccdB</td><td>306</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245048 BBa_K245048]</td><td>Intermediate</td><td>H1-P2</td><td>204</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245049 BBa_K245049]</td><td>Intermediate</td><td>KSI-DP-P1</td><td>489</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245050 BBa_K245050]</td><td>Composite</td><td>GyrB-CutA1</td><td>996</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245051 BBa_K245051]</td><td>Composite</td><td>GyrB-SG-SG-CutA1</td><td>1002</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245052 BBa_K245052]</td><td>Intermediate</td><td>GCN_P2</td><td>204</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245053 BBa_K245053]</td><td>Intermediate</td><td>P4-H1</td><td>204</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245054 BBa_K245054]</td><td>Intermediate</td><td>P2-H1</td><td>204</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245055 BBa_K245055]</td><td>Intermediate</td><td>P6-H1</td><td>204</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245056 BBa_K245056]</td><td>Intermediate</td><td>P3-P5</td><td>204</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245057 BBa_K245057]</td><td>Intermediate</td><td>P4-P2</td><td>204</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245058 BBa_K245058]</td><td>Composite</td><td>KSI-DP-P6</td><td>489</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245059 BBa_K245059]</td><td>Intermediate</td><td>LL37-ELST</td><td>267</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245060 BBa_K245060]</td><td>Intermediate</td><td>ELST-ELST-ELST</td><td>462</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245061 BBa_K245061]</td><td>Intermediate</td><td>ELST-ELST</td><td>306</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245062 BBa_K245062]</td><td>Composite</td><td>P1-H1-P2</td><td>309</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245063 BBa_K245063]</td><td>Composite</td><td>KSI-DP-P1-H1-P2</td><td>699</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245064 BBa_K245064]</td><td>Composite</td><td>P1-BCR-P2</td><td>318</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245065 BBa_K245065]</td><td>Composite</td><td>P1-APH-P2</td><td>345</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245066 BBa_K245066]</td><td>Composite</td><td>P1-GCN-P2</td><td>309</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245067 BBa_K245067]</td><td>Composite</td><td>P3-P4-H1</td><td>309</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245068 BBa_K245068]</td><td>Composite</td><td>P1-P3-P5</td><td>309</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245069 BBa_K245069]</td><td>Composite</td><td>KSI-DP-P1-P3-P5</td><td>699</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245070 BBa_K245070]</td><td>Composite</td><td>P6-P4-P2</td><td>309</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245071 BBa_K245071]</td><td>Composite</td><td>KSI-DP-P6-P4-P2</td><td>699</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245072 BBa_K245072]</td><td>Composite</td><td>KSI-DP-LL37-ELST</td><td>657</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245073 BBa_K245073]</td><td>Composite</td><td>KSI-DP-LL37-ELST-ELST-ELST-ELST</td><td>1125</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245074 BBa_K245074]</td><td>Composite</td><td>KSI-DP-LL-37-ELST-GCN-ELST</td><td>918</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245075 BBa_K245075]</td><td>Composite</td><td>KSI-DP-EEE</td><td>576</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245076 BBa_K245076]</td><td>Composite</td><td>KSI_DP-LL37-ELST-P2-ELST</td><td>918</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245077 BBa_K245077]</td><td>Composite</td><td>KSI-DP-LL37-ELST-P4-ELST</td><td>918</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245078 BBa_K245078]</td><td>Composite</td><td>LL37-ELST-GCN-ELST</td><td>528</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245079 BBa_K245079]</td><td>Composite</td><td>GCN-ELST</td><td>255</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245080 BBa_K245080]</td><td>DNA</td><td>scar Xba/Spe NgoMIV</td><td>12</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245081 BBa_K245081]</td><td>Composite</td><td>P2-ELST</td><td>255</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245082 BBa_K245082]</td><td>Composite</td><td>P4-ELST</td><td>255</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245083 BBa_K245083]</td><td>Composite</td><td>TA-P1</td><td>168</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245084 BBa_K245084]</td><td>Composite</td><td>TB-P3</td><td>168</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245085 BBa_K245085]</td><td>Composite</td><td>TC-P5</td><td>168</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245086 BBa_K245086]</td><td>Composite</td><td>P2-TA</td><td>168</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245087 BBa_K245087]</td><td>Composite</td><td>P4-TB</td><td>168</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245088 BBa_K245088]</td><td>Composite</td><td>TC-P5</td><td>168</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245089 BBa_K245089]</td><td>Composite</td><td>foldon-APH</td><td>222</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245090 BBa_K245090]</td><td>Composite</td><td>foldon-BCR</td><td>195</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245091 BBa_K245091]</td><td>Composite</td><td>p53-APH1</td><td>186</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245092 BBa_K245092]</td><td>Composite</td><td>p53-APH</td><td>231</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245093 BBa_K245093]</td><td>Composite</td><td>p53-BCR</td><td>204</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245094 BBa_K245094]</td><td>Composite</td><td>foldon-p53</td><td>177</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245095 BBa_K245095]</td><td>Composite</td><td>GyrB-p53</td><td>753</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245096 BBa_K245096]</td><td>Intermediate</td><td>BCR-P2</td><td>213</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245097 BBa_K245097]</td><td>Intermediate</td><td>APH-P2</td><td>240</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245098 BBa_K245098]</td><td>Composite</td><td>GyrB-foldon</td><td>744</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245099 BBa_K245099]</td><td>Composite</td><td>APH-p53</td><td>231</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245100 BBa_K245100]</td><td>Composite</td><td>BCR-p53</td><td>204</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245101 BBa_K245101]</td><td>Composite</td><td>LL37-ELST-GCN-ELST-P1</td><td>633</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245102 BBa_K245102]</td><td>Composite</td><td>LL37-ELST-GCN-ELST-P2</td><td>633</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245105 BBa_K245105]</td><td>Composite</td><td>ELST-ELST-ELST-mNGF</td><td>1137</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245106 BBa_K245106]</td><td>Composite</td><td>RADA-mNGF</td><td>741</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245107 BBa_K245107]</td><td>Composite</td><td>LL37-ELST-GCN-ELST-mNGF</td><td>1203</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245108 BBa_K245108]</td><td>Composite</td><td>KSI-DP-P2/33-LL37-ELST-GCN-ELST</td><td>945</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245111 BBa_K245111]</td><td>Composite</td><td>P2-P6-H1</td><td>309</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245112 BBa_K245112]</td><td>Composite</td><td>P5-P2-H1</td><td>309</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245113 BBa_K245113]</td><td>Coding</td><td>KSI-DP</td><td>378</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245114 BBa_K245114]</td><td>Coding</td><td>LL37</td><td>111</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245115 BBa_K245115]</td><td>Coding</td><td>KSI-DP-P2/33</td><td>405</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245116 BBa_K245116]</td><td>Coding</td><td>GyrB</td><td>657</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245117 BBa_K245117]</td><td>Coding</td><td>GyrB-SG</td><td>663</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245118 BBa_K245118]</td><td>Coding</td><td>P1</td><td>99</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245119 BBa_K245119]</td><td>Coding</td><td>P2</td><td>99</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245120 BBa_K245120]</td><td>Coding</td><td>P3</td><td>99</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245121 BBa_K245121]</td><td>Coding</td><td>P4</td><td>99</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245122 BBa_K245122]</td><td>Coding</td><td>P5</td><td>99</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245123 BBa_K245123]</td><td>Coding</td><td>P6</td><td>99</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245124 BBa_K245124]</td><td>Coding</td><td>P7</td><td>99</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245125 BBa_K245125]</td><td>Coding</td><td>P8</td><td>99</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245126 BBa_K245126]</td><td>Coding</td><td>H1</td><td>99</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245127 BBa_K245127]</td><td>Coding</td><td>GCN</td><td>99</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245128 BBa_K245128]</td><td>Coding</td><td>p53</td><td>90</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245129 BBa_K245129]</td><td>Coding</td><td>CutA1</td><td>333</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245130 BBa_K245130]</td><td>Coding</td><td>T1W_AEA</td><td>186</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245131 BBa_K245131]</td><td>Coding</td><td>ELST</td><td>150</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245132 BBa_K245132]</td><td>Coding</td><td>APH1</td><td>90</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245133 BBa_K245133]</td><td>Coding</td><td>APH</td><td>135</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245134 BBa_K245134]</td><td>Coding</td><td>BCR</td><td>108</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245135 BBa_K245135]</td><td>Coding</td><td>APHE</td><td>135</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245139 BBa_K245139]</td><td>Coding</td><td>RADA</td><td>66</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245140 BBa_K245140]</td><td>Coding</td><td>mNGF</td><td>669</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245141 BBa_K245141]</td><td>Coding</td><td>foldon</td><td>81</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245200 BBa_K245200]</td><td>Composite</td><td>P1-P4-H1</td><td>309</td></tr> | ||
| + | </table> | ||
| + | |||
| + | }} | ||
Latest revision as of 02:33, 22 October 2009
|
|
Designed BioBricksWe have designed four NEW functionalised linker-extension standard vectors. All together, 106 parts were generated all cloned into our NEW functionalised linker-extension standard vectors and forty-one were single coding, thirteen intermediates and forty-eight composite parts. Plasmid backbonesFour NEW linker-extension standard vectors are constructed.
The coding sequencesThe forty-one BioBrick coding sequences are prepared and are listed as (i) coiled-coil-forming segments, (ii) oligimerisation domains, (iii) functional domains and others.
Coiled-coil segments
Oligomerization domains
Functional domains
Others
The intermediate sequencesThirteen BioBricks with intermediate parts are constructed.
Composite partsThe forty-eight BioBricks as composites are prepared, which are used in (i) polypeptide manufacturing, (ii) polypeptide membranes, (iii) coiled-coil polyhedra, (iv) regulated assembly and (v) functional biopolymers.
Polypeptide manufacturing
Polypeptide membrane
Coiled-coil polyhedra
Regulated assembly
Functional biopolymers
Slovenia 2009 iGEM Team Parts Sandbox
|
