Team:Kyoto/CiC/Notebook/0907-0911

(Difference between revisions)

Latest revision as of 16:15, 21 October 2009

Colony was observed (090912)　

@@ Line 33: / Line 33: @@
 ===Wednesday, 16 September===
 ====To Do====
-*PCR
+*HIV-TAT-(LALAAAA)3
-**HIV-TAT-(LALAAAA)3
+**PCR
-*Restriction Enzyme Digestion
+**Restriction Enzyme Digestion(EcoR1 + Spe1)
-**HIV-TAT-(LALAAAA)3 (EcoR1 + Spe1)
+*B0015
-**B0015 (EcoR1 + Spe1)
+**Restriction Enzyme Digestion(EcoR1 + Xba1)
-*Electrophoresis
 ====Results====
+*Band of expected size were observed.
+{{:Team:Kyoto/Pad_between_dates}}
-===Thursday, 17 September===
+===Fryday, 11 September===
 ====To Do====
-*Make repetitive sequence (MPR)
+*HIV-TAT-(LALAAAA)3
-*Transformation(retry)
+**Ligation
-{{:Team:Kyoto/Pad_endof_dates}}
+**Transformation
-<html></div><!-- /#rightcontents --></html>
+====Results====
+=====ligation=====
+{| class="table"
+|+conc.
+!NO.!!Sample name!!conc./(ng/ul)
+|-
+|1||HIV-TAT sample1(EcoR1,Spe1)||36.2
+|-
+|2||HIV-TAT sample2(EcoR1,Spe1)||21.7
+|-
+|3||HIV-TAT sample3(EcoR1,Spe1)||47.9
+|-
+|4||B0015(EcoR1,Xba1)||2.48
+|}
+=====Transformation=====
+Colony was observed (090912)
+{{:Team:Kyoto/Pad_between_dates}}