Team:Kyoto/CiC/Notebook/0907-0911
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**PCR | **PCR | ||
**Restriction Enzyme Digestion(EcoR1 + Spe1) | **Restriction Enzyme Digestion(EcoR1 + Spe1) | ||
- | |||
*B0015 | *B0015 | ||
**Restriction Enzyme Digestion(EcoR1 + Xba1) | **Restriction Enzyme Digestion(EcoR1 + Xba1) | ||
- | |||
====Results==== | ====Results==== | ||
- | + | *Band of expected size were observed. | |
{{:Team:Kyoto/Pad_between_dates}} | {{:Team:Kyoto/Pad_between_dates}} | ||
===Fryday, 11 September=== | ===Fryday, 11 September=== | ||
====To Do==== | ====To Do==== | ||
- | *Ligation | + | *HIV-TAT-(LALAAAA)3 |
- | *Transformation | + | **Ligation |
+ | **Transformation | ||
====Results==== | ====Results==== | ||
=====ligation===== | =====ligation===== | ||
Line 55: | Line 54: | ||
!NO.!!Sample name!!conc./(ng/ul) | !NO.!!Sample name!!conc./(ng/ul) | ||
|- | |- | ||
- | |1||HIV-TAT | + | |1||HIV-TAT sample1(EcoR1,Spe1)||36.2 |
|- | |- | ||
- | |2||HIV-TAT | + | |2||HIV-TAT sample2(EcoR1,Spe1)||21.7 |
|- | |- | ||
- | |3||HIV-TAT | + | |3||HIV-TAT sample3(EcoR1,Spe1)||47.9 |
|- | |- | ||
|4||B0015(EcoR1,Xba1)||2.48 | |4||B0015(EcoR1,Xba1)||2.48 |
Latest revision as of 16:15, 21 October 2009
- 0907-0911
- constructing HIV-TAT-(LALAAAA)3-generator
- 0914-0918
- constructing HIV-TAT-(LALAAAA)3-generator
- 0921-0925
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 0928-1002
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1005-1009
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1012-1016
- Observation
- 1019-1023
- Observation
097-0911 : Plasmid Construction of HIV-TAT-(LALAAAA)3-generator
Wednesday, 16 September
To Do
- HIV-TAT-(LALAAAA)3
- PCR
- Restriction Enzyme Digestion(EcoR1 + Spe1)
- B0015
- Restriction Enzyme Digestion(EcoR1 + Xba1)
Results
- Band of expected size were observed.
Fryday, 11 September
To Do
- HIV-TAT-(LALAAAA)3
- Ligation
- Transformation
Results
ligation
NO. | Sample name | conc./(ng/ul) |
---|---|---|
1 | HIV-TAT sample1(EcoR1,Spe1) | 36.2 |
2 | HIV-TAT sample2(EcoR1,Spe1) | 21.7 |
3 | HIV-TAT sample3(EcoR1,Spe1) | 47.9 |
4 | B0015(EcoR1,Xba1) | 2.48 |
Transformation
Colony was observed (090912)