Team:Kyoto/CiC/Notebook/0921-0925

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==0914-0918 : what to do. what to do. what to do. what to do. ==
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==0914-0918 : constructing sig-GFP generator,sig-EGFP generator(HeLa)  ==
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===Monday, 21 September===
===Monday, 21 September===
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**Colony PCR
**Colony PCR
====Results====
====Results====
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*消えた
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*Binds of expected size were observed.
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===Tuesday, 21 September===
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===Tuesday, 22 September===
====To Do====
====To Do====
*HIV-TAT-(LALAAAA)3-generator
*HIV-TAT-(LALAAAA)3-generator
-
**Colony PCR
+
**miniprep
 +
*sig-EGFP generator(HeLa)
 +
**Restriction Enzyme PEGFP-N3 VECTOR (EGFP generator)
 +
**hosphorylation signal sequence primer
 +
**annering signal sequence primer
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===Wednesday, 16 September===
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===Wednesday, 23 September===
====To Do====
====To Do====
 +
*sig-EGFP generator
**ligation
**ligation
**transformation
**transformation
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{| class="table"
{| class="table"
|+conc.
|+conc.
-
!NO.!!Sample name!!conc./(ng/ul)
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!NO.!!Sample name!!conc./(ng/ul)!!conc.(ul)
 +
|-
 +
|1||pEGFP-N3(EcoR1,Xho1)||224.3||1
|-
|-
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|1||11(stu1)||103.2
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|2||signal sequence||0.3||11
|}
|}
=====Transformation=====
=====Transformation=====
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No colony was observed (090917 10:00)　
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No colony was observed. (090917 10:00)　
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Latest revision as of 02:06, 22 October 2009

  1. Home
  2. CiC
  3. Notebook

0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

0914-0918 : constructing sig-GFP generator,sig-EGFP generator(HeLa)

Monday, 21 September

To Do

  • HIV-TAT-(LALAAAA)3-generator
    • Colony PCR

Results

  • Binds of expected size were observed.

Tuesday, 22 September

To Do

  • HIV-TAT-(LALAAAA)3-generator
    • miniprep
  • sig-EGFP generator(HeLa)
    • Restriction Enzyme PEGFP-N3 VECTOR (EGFP generator)
    • hosphorylation signal sequence primer
    • annering signal sequence primer

Wednesday, 23 September

To Do

  • sig-EGFP generator
    • ligation
    • transformation

Results

ligation
conc.
NO.Sample nameconc./(ng/ul)conc.(ul)
1pEGFP-N3(EcoR1,Xho1)224.31
2signal sequence0.311
Transformation

No colony was observed. (090917 10:00)　

Thursday, 17 September

To Do

  • Make repetitive sequence (MPR)
  • Transformation(retry)

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