Team:Kyoto/CiC/Notebook/0921-0925

(Difference between revisions)

Latest revision as of 02:06, 22 October 2009

HIV-TAT-(LALAAAA)3-generator
- miniprep
sig-EGFP generator(HeLa)
- Restriction Enzyme PEGFP-N3 VECTOR (EGFP generator)
- hosphorylation signal sequence primer
- annering signal sequence primer

conc.
NO.	Sample name	conc./(ng/ul)	conc.(ul)
1	pEGFP-N3(EcoR1,Xho1)	224.3	1
2	signal sequence	0.3	11

No colony was observed. (090917 10:00)　

@@ Line 29: / Line 29: @@
 </html>
 {{:Team:Kyoto/CiC/Notebook_tpl}}
-==0914-0918 : what to do. what to do. what to do. what to do. ==
+==0914-0918 : constructing sig-GFP generator,sig-EGFP generator(HeLa)  ==
 {{:Team:Kyoto/Pad_start_dates}}
 ===Monday, 21 September===
 ====To Do====
-*No experiments were undertaken
+*HIV-TAT-(LALAAAA)3-generator
+**Colony PCR
+====Results====
+*Binds of expected size were observed.
 {{:Team:Kyoto/Pad_between_dates}}
@@ Line 40: / Line 43: @@
 *HIV-TAT-(LALAAAA)3-generator
 **miniprep
-*TIM23complex signal::EGFP generator
+*sig-EGFP generator(HeLa)
-**PEGFP-N3 VECTOR (EGFP generator)
+**Restriction Enzyme PEGFP-N3 VECTOR (EGFP generator)
-====Results====
+**hosphorylation signal sequence primer
-*消えた
+**annering signal sequence primer
 {{:Team:Kyoto/Pad_between_dates}}
 ===Wednesday, 23 September===
 ====To Do====
+*sig-EGFP generator
 **ligation
 **transformation
@@ Line 55: / Line 60: @@
 {| class="table"
 |+conc.
-!NO.!!Sample name!!conc./(ng/ul)
+!NO.!!Sample name!!conc./(ng/ul)!!conc.(ul)
+|-
+|1||pEGFP-N3(EcoR1,Xho1)||224.3||1
 |-
-|1||11(stu1)||103.2
+|2||signal sequence||0.3||11
 |}
 =====Transformation=====
-No colony was observed (090917 10:00)
+No colony was observed. (090917 10:00)
 {{:Team:Kyoto/Pad_between_dates}}