SNOWDRIFT/Proof of concept

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Latest revision as of 02:33, 22 October 2009

Proof of Concept

In order to establish cell-cell communication protein signalling can come in handy. We attempted to do this by attaching what we call a 'secretion tag' to our protein of interest. This 'tag' allows secretion of proteins.

This tag is designed by using N-terminal sequences from proteins which are exported using the TAT Pathway.

We tried to test this export mechanism using the following biobricks:

Both these constructs are designed to secrete the Green Fluorescent Protein in to the extracellular environment.

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-'''Proof of Concept:'''
+{{Team:IBB_Pune/header}}
+{{Team:IBB_Pune/menu}}
+<br>
+<p><span style="font-weight:bold; font-size:200%; color:#0000cc;">Proof of Concept</span></p>
+<br>
 In order to establish cell-cell communication protein signalling can come in handy.
 We attempted to do this by attaching what we call a 'secretion tag' to our protein of interest. This 'tag' allows secretion of proteins.
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 We tried to test this export mechanism using the following biobricks:
 <br>
-[[Image:Snowdriftproofofconcept.JPG]]
+[[Image:Snowdriftproofofconcept.JPG|center]]
 <br>
 Both these constructs are designed to secrete the Green Fluorescent Protein in to the extracellular environment.
+<html>
+<p><a href="https://2009.igem.org/SNOWDRIFT/our_idea
+ "><span style="font-weight:bold; font-size:150%; color:#0000cc;"><u>Next</u></span></a></p>
+</html>