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SNOWDRIFT/Proof of concept
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| - | <p><span style="font-weight:bold; font-size:200%; color:# | + | <p><span style="font-weight:bold; font-size:200%; color:#0000cc;">Proof of Concept</span></p> |
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In order to establish cell-cell communication protein signalling can come in handy. | In order to establish cell-cell communication protein signalling can come in handy. | ||
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We tried to test this export mechanism using the following biobricks: | We tried to test this export mechanism using the following biobricks: | ||
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Both these constructs are designed to secrete the Green Fluorescent Protein in to the extracellular environment. | Both these constructs are designed to secrete the Green Fluorescent Protein in to the extracellular environment. | ||
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Latest revision as of 02:33, 22 October 2009
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Proof of Concept
In order to establish cell-cell communication protein signalling can come in handy. We attempted to do this by attaching what we call a 'secretion tag' to our protein of interest. This 'tag' allows secretion of proteins.This tag is designed by using N-terminal sequences from proteins which are exported using the TAT Pathway.
We tried to test this export mechanism using the following biobricks:
Both these constructs are designed to secrete the Green Fluorescent Protein in to the extracellular environment.
