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Deposited BioBricks.html
From 2009.igem.org
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__NOTOC__ | __NOTOC__ | ||
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| - | =Designed BioBricks= | + | <html><center> |
| + | <font size="6" color="#009ee0"><b>Deposited BioBricks</b></font> | ||
| + | </center></html> | ||
| + | <br> | ||
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| + | ==Designed BioBricks== | ||
We have designed four NEW functionalised linker-extension standard vectors. All together, 106 parts were generated all cloned into our NEW functionalised linker-extension standard vectors and forty-one were single coding, thirteen intermediates and forty-eight composite parts. | We have designed four NEW functionalised linker-extension standard vectors. All together, 106 parts were generated all cloned into our NEW functionalised linker-extension standard vectors and forty-one were single coding, thirteen intermediates and forty-eight composite parts. | ||
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==The coding sequences== | ==The coding sequences== | ||
The forty-one BioBrick coding sequences are prepared and are listed as (i) coiled-coil-forming segments, (ii) oligimerisation domains, (iii) functional domains and others. | The forty-one BioBrick coding sequences are prepared and are listed as (i) coiled-coil-forming segments, (ii) oligimerisation domains, (iii) functional domains and others. | ||
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===Coiled-coil segments=== | ===Coiled-coil segments=== | ||
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<table class="crte"> | <table class="crte"> | ||
<tr><td><b>Name</b></td><td><b>Type</b></td><td><b>Description</b></td><td><b>Length</b></td></tr> | <tr><td><b>Name</b></td><td><b>Type</b></td><td><b>Description</b></td><td><b>Length</b></td></tr> | ||
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</table> | </table> | ||
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<br> | <br> | ||
===Oligomerization domains=== | ===Oligomerization domains=== | ||
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<br> | <br> | ||
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===Functional domains=== | ===Functional domains=== | ||
<table class="crte"> | <table class="crte"> | ||
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===Others=== | ===Others=== | ||
<table class="crte"> | <table class="crte"> | ||
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<br> | <br> | ||
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==The intermediate sequences== | ==The intermediate sequences== | ||
Thirteen BioBricks with intermediate parts are constructed. | Thirteen BioBricks with intermediate parts are constructed. | ||
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<br> | <br> | ||
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===Polypeptide manufacturing=== | ===Polypeptide manufacturing=== | ||
<table class="crte"> | <table class="crte"> | ||
<tr><td><b>Name</b></td><td><b>Type</b></td><td><b>Description</b></td><td><b>Length</b></td></tr> | <tr><td><b>Name</b></td><td><b>Type</b></td><td><b>Description</b></td><td><b>Length</b></td></tr> | ||
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245115 BBa_K245115]</td><td>Coding</td><td>KSI-DP-P2/33</td><td>405</td></tr> | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245115 BBa_K245115]</td><td>Coding</td><td>KSI-DP-P2/33</td><td>405</td></tr> | ||
| - | + | </table> | |
<br> | <br> | ||
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===Polypeptide membrane=== | ===Polypeptide membrane=== | ||
<table class="crte"> | <table class="crte"> | ||
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<br> | <br> | ||
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===Coiled-coil polyhedra=== | ===Coiled-coil polyhedra=== | ||
<table class="crte"> | <table class="crte"> | ||
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<br> | <br> | ||
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===Regulated assembly=== | ===Regulated assembly=== | ||
<table class="crte"> | <table class="crte"> | ||
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<br> | <br> | ||
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===Functional biopolymers=== | ===Functional biopolymers=== | ||
<table class="crte"> | <table class="crte"> | ||
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<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245007 BBa_K245007]</td><td>Plasmid_Backbone</td><td>BB-NIC-II-HisC</td><td>2167</td></tr> | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245007 BBa_K245007]</td><td>Plasmid_Backbone</td><td>BB-NIC-II-HisC</td><td>2167</td></tr> | ||
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245008 BBa_K245008]</td><td>Plasmid_Backbone</td><td>BB-NIC-III-HisN</td><td>2261</td></tr> | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245008 BBa_K245008]</td><td>Plasmid_Backbone</td><td>BB-NIC-III-HisN</td><td>2261</td></tr> | ||
| - | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245009 BBa_K245009]</td><td>DNA</td><td>RFC 37 Scar Sequence</td><td>6</td></tr> | + | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245009 BBa_K245009]</td><td>DNA</td><td><nowiki>RFC</nowiki> 37 Scar Sequence</td><td>6</td></tr> |
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245021 BBa_K245021]</td><td>Coding</td><td>cYFP</td><td>252</td></tr> | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245021 BBa_K245021]</td><td>Coding</td><td>cYFP</td><td>252</td></tr> | ||
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245022 BBa_K245022]</td><td>Coding</td><td>nYFP</td><td>462 </td></tr> | <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245022 BBa_K245022]</td><td>Coding</td><td>nYFP</td><td>462 </td></tr> | ||
Latest revision as of 02:33, 22 October 2009
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Designed BioBricksWe have designed four NEW functionalised linker-extension standard vectors. All together, 106 parts were generated all cloned into our NEW functionalised linker-extension standard vectors and forty-one were single coding, thirteen intermediates and forty-eight composite parts. Plasmid backbonesFour NEW linker-extension standard vectors are constructed.
The coding sequencesThe forty-one BioBrick coding sequences are prepared and are listed as (i) coiled-coil-forming segments, (ii) oligimerisation domains, (iii) functional domains and others.
Coiled-coil segments
Oligomerization domains
Functional domains
Others
The intermediate sequencesThirteen BioBricks with intermediate parts are constructed.
Composite partsThe forty-eight BioBricks as composites are prepared, which are used in (i) polypeptide manufacturing, (ii) polypeptide membranes, (iii) coiled-coil polyhedra, (iv) regulated assembly and (v) functional biopolymers.
Polypeptide manufacturing
Polypeptide membrane
Coiled-coil polyhedra
Regulated assembly
Functional biopolymers
Slovenia 2009 iGEM Team Parts Sandbox
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