Team:UNICAMP-Brazil/Notebooks/October 14
From 2009.igem.org
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''Fabi and Léo'' | ''Fabi and Léo'' | ||
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+ | ==== Confirming the New Device ==== | ||
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+ | *<p style=”text-align:justify;”>In order to confirm definitively our new part, we made two tests. We made PCR with VF2 and VR primers and we made enzyme restriction analysis with EcoRI and PstI. After run the agarose gel, we finally confirm our new part. We expected 840 bp (for PCR) and 670 pb (Restriction enzyme). We get it!!! Yes!! [[Image:New_biobrick.JPG|500px|center]] | ||
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+ | ''Luige, Ane and Marcos'' | ||
==''' YeastGuard '''== | ==''' YeastGuard '''== | ||
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====[https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/pGEMStrategy New Strategy: pGEM]==== | ====[https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/pGEMStrategy New Strategy: pGEM]==== | ||
- | * | + | * We did miniprep of 10 ''ADH1''+Lysozyme colonies, then we digested the ''ADH1''+Lysozyme/biofusion minipreps in order to connect it to the yeast expression vector (YEP358). We used the ''Xba''I and ''Pst''I enzymes. 9 of 10 digestions worked! =) The expected fragment size is 2000bp. |
- | + | [[Image:20091021_confirmacao_adh1lys_results.png|300px|center]] | |
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- | * | + | * We chose two fragments and purified them from the agarose gel. We ligated them to YEP358. We will transform it tomorrow. |
Latest revision as of 03:47, 22 October 2009
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