Team:UNICAMP-Brazil/Notebooks/October 13
From 2009.igem.org
(4 intermediate revisions not shown) | |||
Line 4: | Line 4: | ||
==''' ColiGuard '''== | ==''' ColiGuard '''== | ||
+ | |||
+ | ====Cre-Recombinase - Final Conclusions==== | ||
+ | |||
+ | *<p style=”text-align:justify;”>Today is October 13th... We intend to already send our assembled biobricks this Thursday (October 15th). We also have less then 10 days to update and finish this entire wiki, and to conclude our mainly experiments.</p> | ||
+ | *<p style=”text-align:justify;”>Therefore, our team decided that we are going to leave aside our Cre's device construction. We already got the part's biobricks, and assembling the entire device will demand a lot of time, and time is kind of lacking right now... =]</p> | ||
+ | *<p style=”text-align:justify;”>It's already a great achievement to successful assemble Cre-Recombinase without it's ATG start codon. So, we are truly satisfied that we actually did it!</p> | ||
+ | *<p style=”text-align:justify;”>Time to work on other stuffs now...</p> | ||
+ | |||
+ | ''Víctor'' | ||
==== PY Promoter - Mini-prep and restriction analysis ==== | ==== PY Promoter - Mini-prep and restriction analysis ==== | ||
Line 17: | Line 26: | ||
*<p style=”text-align:justify;”>As we can observe in the gel photo, all plasmids presented bands compatible with the expected size for the digestion.</p> | *<p style=”text-align:justify;”>As we can observe in the gel photo, all plasmids presented bands compatible with the expected size for the digestion.</p> | ||
- | *<p style=”text-align:justify;”>Now that we confirmed that our construction in biobrick format is right they are ready to be | + | *<p style=”text-align:justify;”>Now that we have confirmed that our construction in biobrick format is right they are ready to be submitted to the registry! =)</p> |
*<p style=”text-align:justify;”>We decided to send the PY1 promoter inserted in plasmid BBa_J23100. It is our biobrick [http://partsregistry.org/wiki/index.php?title=Part:BBa_K284008 BBa_K284008]</p> | *<p style=”text-align:justify;”>We decided to send the PY1 promoter inserted in plasmid BBa_J23100. It is our biobrick [http://partsregistry.org/wiki/index.php?title=Part:BBa_K284008 BBa_K284008]</p> | ||
Line 23: | Line 32: | ||
''Fabi and Léo'' | ''Fabi and Léo'' | ||
- | + | ==== PCR colony. New device ==== | |
- | ==== | + | |
We found transforming colonies apparently. So, we made colony PCR in order to prove the correct transformation. We used the VF2 and VR primers. We run the agarose gel and???………Yess!!! we get!!!. We observed some samples with band size expected (~840bp) | We found transforming colonies apparently. So, we made colony PCR in order to prove the correct transformation. We used the VF2 and VR primers. We run the agarose gel and???………Yess!!! we get!!!. We observed some samples with band size expected (~840bp) |
Latest revision as of 03:51, 22 October 2009
|