Team:UNICAMP-Brazil/Notebooks/October 20
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====Yeast experiments: Lysozyme==== | ====Yeast experiments: Lysozyme==== | ||
- | *<p style=”text-align:justify;”>Today we began the tests with the yeasts transformed with the following constructions: YEP+pDLD-Lysozyme and YEP+ | + | *<p style=”text-align:justify;”>Today we began the tests with the yeasts transformed with the following constructions: YEP+pDLD-Lysozyme and YEP+''ADH1''-Lysozyme.</p> |
* The pre inocula made yesterday grew. | * The pre inocula made yesterday grew. | ||
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* We will have 10 samples at the end of the tests: | * We will have 10 samples at the end of the tests: | ||
a) Wild type: inoculated in Ura+ media. No plasmid, no induction, no lysozyme expression. | a) Wild type: inoculated in Ura+ media. No plasmid, no induction, no lysozyme expression. | ||
- | b) Glucose | + | |
+ | b) Glucose ''ADH1''+Lys: plasmid with lysozyme under control of ''ADH1'', a constitutive promoter. No induction. | ||
+ | |||
c) Glucose pDLD+Lys: No induction. Lysozyme under control of pDLD, a lactate responsive promoter. | c) Glucose pDLD+Lys: No induction. Lysozyme under control of pDLD, a lactate responsive promoter. | ||
+ | |||
d) Glucose pDLD+Lys 1: lysozyme under control of pDLD, a lactate responsive promoter. Induction with 5mM lactate. | d) Glucose pDLD+Lys 1: lysozyme under control of pDLD, a lactate responsive promoter. Induction with 5mM lactate. | ||
+ | |||
e) Glucose pDLD+Lys 2: lysozyme under control of pDLD, a lactate responsive promoter. Induction with 25mM lactate. | e) Glucose pDLD+Lys 2: lysozyme under control of pDLD, a lactate responsive promoter. Induction with 25mM lactate. | ||
+ | |||
f) Glucose pDLD+Lys 3: lysozyme under control of pDLD, a lactate responsive promoter. Induction with 50mM lactate | f) Glucose pDLD+Lys 3: lysozyme under control of pDLD, a lactate responsive promoter. Induction with 50mM lactate | ||
+ | |||
g) Ethanol pDLD1+Lys: No induction. Lysozyme under control of Adh1, a constitutive promoter. | g) Ethanol pDLD1+Lys: No induction. Lysozyme under control of Adh1, a constitutive promoter. | ||
+ | |||
h) Ethanol pDLD1+Lys 1: lysozyme under control of pDLD, a lactate responsive promoter. Induction with 5mM lactate. | h) Ethanol pDLD1+Lys 1: lysozyme under control of pDLD, a lactate responsive promoter. Induction with 5mM lactate. | ||
- | i) Ethanol pDLD1+Lys 2: lysozyme under control of pDLD, a lactate responsive promoter. Induction with 25mM lactate | + | |
+ | i) Ethanol pDLD1+Lys 2: lysozyme under control of pDLD, a lactate responsive promoter. Induction with 25mM lactate. | ||
+ | |||
j) Ethanol pDLD1+Lys 3: lysozyme under control of pDLD, a lactate responsive promoter. Induction with 50mM lactate | j) Ethanol pDLD1+Lys 3: lysozyme under control of pDLD, a lactate responsive promoter. Induction with 50mM lactate | ||
Latest revision as of 03:33, 22 October 2009
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