Team:UNICAMP-Brazil/Notebooks/October 20
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====Yeast experiments: Lysozyme==== | ====Yeast experiments: Lysozyme==== | ||
- | *<p style=”text-align:justify;”>Today we began the tests with the yeasts transformed with the following constructions: YEP+pDLD-Lysozyme and YEP+ | + | *<p style=”text-align:justify;”>Today we began the tests with the yeasts transformed with the following constructions: YEP+pDLD-Lysozyme and YEP+''ADH1''-Lysozyme.</p> |
* The pre inocula made yesterday grew. | * The pre inocula made yesterday grew. | ||
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a) Wild type: inoculated in Ura+ media. No plasmid, no induction, no lysozyme expression. | a) Wild type: inoculated in Ura+ media. No plasmid, no induction, no lysozyme expression. | ||
- | b) Glucose | + | b) Glucose ''ADH1''+Lys: plasmid with lysozyme under control of ''ADH1'', a constitutive promoter. No induction. |
c) Glucose pDLD+Lys: No induction. Lysozyme under control of pDLD, a lactate responsive promoter. | c) Glucose pDLD+Lys: No induction. Lysozyme under control of pDLD, a lactate responsive promoter. |
Latest revision as of 03:33, 22 October 2009
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