Team:IBB Pune/project/Results

From 2009.igem.org

(Difference between revisions)

Latest revision as of 02:44, 22 October 2009

Characterization of And Gates:

Even as the wiki freezes we are characterizing Const.Prom-LacO-mRFP for its expression. We hope we get positive results.

We carried out the following transformations successfully:

Native Biobricks

pLL promoter - BBa_I746365
GFP coding sequence - BBa_E0040
RFP coding sequence
GFP Translational unit
RFP Translational unit
Enhanced CFP
Fast GFP
Constitutive promoter -BBa_J23102
Promoter + RBS
T7 promoter
pLuxR
Double terminator
RBS
Constitutive LuxI
LuxR Translational unit

OUR PARTS

YcdB
TorA
Lac Operator

Assembly

Const. Promoter-LacO
PluxR- LuxI
{Promoter + RBS}-GFP
{Promoter + RBS}-TorA
{Promoter + RBS}-Ycdb
luxR-PluxR-{RBS-LuxI}
Promoter + RBS
YcdB-GFP
TorA-GFP
{Promoter + RBS}-LuxR
{Promoter + RBS}-YcdB-GFP
{Promoter + RBS}-TorA-GFP
Const. Promoter-LuxR

Failed Transformations

Backbones

pSB1AK3 with CcdB gene
pSB1T3 with CcdB gene
pSB1C3 with CcdB gene
pSB1K3 with CcdB gene
pSB1A3 with CcdB gene
pSB1AC3 with CcdB gene
pSB1AK3 with mRFP
pSB1T3 with mRFP
pSB1C3 with mRFP
pSB1K3 with mRFP
pSB1A3 with mRFP
pSB1AC3 with mRFP

Native Biobricks

Lysis Cassette
Constitutive GFP
COnstitutive CFP

GEL PICTURES

Lane1	Lane2	Lane3	Lane4	Lane5	Lane6	Lane7
TorA	YcdB	YcdB	LacO	LacO	pSB1AK3	pSB1AK3

@@ Line 2: / Line 2: @@
 {{Team:IBB_Pune/menu}}
+<br>
+<p><span style="font-weight:bold; font-size:200%; color:#0000cc;">Characterization of And Gates:</span></p><br>
+Even as the wiki freezes we are characterizing Const.Prom-LacO-mRFP for its expression. We hope we get positive results.
-We carried out the following transformations successfully:
+<br>
+<p><span style="font-weight:bold; font-size:200%; color:#0000cc;">We carried out the following transformations successfully:</span></p><br>
+<p><span style="font-weight:bold; font-size:150%; color:#FF6600;">Native Biobricks</span></p>
-==Native Biobricks==
 # pLL promoter               - BBa_I746365
@@ Line 23: / Line 28: @@
 # LuxR Translational unit
-==OUR PARTS==
+<p><span style="font-weight:bold; font-size:150%; color:#FF6600;">OUR PARTS</span></p>
 # YcdB
@@ Line 30: / Line 36: @@
-==Assembly==
+<p><span style="font-weight:bold; font-size:150%; color:#FF6600;">Assembly</span></p>
 # Const. Promoter-LacO
@@ Line 45: / Line 52: @@
 # {Promoter + RBS}-TorA-GFP
 # Const. Promoter-LuxR
-#
+<p><span style="font-weight:bold; font-size:175%; color:#FF6600;">Failed Transformations</span></p>
+<p><span style="font-weight:bold; font-size:150; color:#FF6600;">Backbones</span></p>
+# pSB1AK3 with CcdB gene
+# pSB1T3 with CcdB gene
+# pSB1C3 with CcdB gene
+# pSB1K3 with CcdB gene
+# pSB1A3 with CcdB gene
+# pSB1AC3 with CcdB gene
+# pSB1AK3 with mRFP
+# pSB1T3 with mRFP
+# pSB1C3 with mRFP
+# pSB1K3 with mRFP
+# pSB1A3 with mRFP
+# pSB1AC3 with mRFP
+<p><span style="font-weight:bold; font-size:150; color:#FF6600;">Native Biobricks</span></p>
+# Lysis Cassette
+# Constitutive GFP
+# COnstitutive CFP
+<p><span style="font-weight:bold; font-size:150; color:#FF6600;">GEL PICTURES</span></p>
+[[Image:10sepgel.jpg|center|500px]]
+<br><br>
+[[Image:14octgel.jpg|center|500px]]
+<html>
+<table border="0" align="center">
+<tr><td>Lane1</td><td>Lane2</td><td>Lane3</td> <td>Lane4</td><td>Lane5</td><td>Lane6</td><td>Lane7</td></tr>
+<tr><td>TorA</td><td>YcdB</td><td>YcdB</td><td>LacO</td><td>LacO</td><td>pSB1AK3</td><td>pSB1AK3</td></tr></table>