Team:UNICAMP-Brazil/Notebooks/August 11

(Difference between revisions)

Latest revision as of 00:34, 22 October 2009

Today we selected 4 colonies of each transformation to confirm it. We inoculated them separetely in LB medium containing the appropriate antibiotic and let them grow O/N.

We decided that we won´t need biobrick BBa_J04630, because the other two reporters are OK for our constructions.

We're going to need large amounts of DNA, so we inoculated colonies in liquid media in order to repeat the plasmid extractions of the six biobricks we are really going to use.

Taís

@@ Line 7: / Line 7: @@
 ====Selection of transformants====
-*<p style=”text-align:justify;”>Today we selected 4 colonies of each transformation to confirm it. We inocculated them separetely in LB medium containing the appropriate antibiotic and let them grow O/N.</p>
+*<p style=”text-align:justify;”>Today we selected 4 colonies of each transformation to confirm it. We inoculated them separetely in LB medium containing the appropriate antibiotic and let them grow O/N.</p>
 ==''' YeastGuard '''==
@@ Line 13: / Line 13: @@
 ====Recovering the biobricks====
-* We decided that we won´t need biobrick BBa_J04630, because the other two reporters are ok for our constructions.
+* We decided that we won´t need biobrick BBa_J04630, because the other two reporters are OK for our constructions.
-* We will need large amounts of DNA so we inoculated colonies in liquid media in order to repeated the plasmid extractions of the six biobricks we are really going to use.
+* We're going to need large amounts of DNA, so we inoculated colonies in liquid media in order to repeat the plasmid extractions of the six biobricks we are really going to use.