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Team:UNICAMP-Brazil/Notebooks/August 18
From 2009.igem.org
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====Terminator biobrick==== | ====Terminator biobrick==== | ||
| - | *<p style=”text-align:justify;”> | + | *<p style=”text-align:justify;”>The biobrick BBa_J63002 was ressuspended from the plate and the transformed ''E. coli'' have occupied the whole plates, both LB-AMP and LB-KAN plates. We decided to replicate these colonies in other plates in order to get isolated colonies for the miniprep.</p> |
| - | The biobrick BBa_J63002 was ressuspended from the plate and the transformed ''E. coli'' have occupied the whole plates, both LB-AMP and LB-KAN plates. We decided to replicate these colonies in other plates in order to get isolated colonies for the miniprep.</p> | + | |
''Taís'' | ''Taís'' | ||
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====Enzyme restriction==== | ====Enzyme restriction==== | ||
| - | + | *<p style=”text-align:justify;”>We performed miniprep according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2] and immediately made enzymatic digestion using ''Xba''I and ''Pst''I in order to prove the existing part in the plasmids. We didn’t find the correct band size (514 bp); we just found the linear plasmid size band.</p> | |
[[Image:18_agosto.jpg |center|]] | [[Image:18_agosto.jpg |center|]] | ||
Latest revision as of 01:06, 22 October 2009
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