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Team:IBB Pune/project/Results
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{{Team:IBB_Pune/menu}} | {{Team:IBB_Pune/menu}} | ||
| + | <br> | ||
| + | <p><span style="font-weight:bold; font-size:200%; color:#0000cc;">Characterization of And Gates:</span></p><br> | ||
| + | Even as the wiki freezes we are characterizing Const.Prom-LacO-mRFP for its expression. We hope we get positive results. | ||
| - | We carried out the following transformations successfully: | + | <br> |
| + | <p><span style="font-weight:bold; font-size:200%; color:#0000cc;">We carried out the following transformations successfully:</span></p><br> | ||
| + | |||
| + | <p><span style="font-weight:bold; font-size:150%; color:#FF6600;">Native Biobricks</span></p> | ||
| - | |||
# pLL promoter - BBa_I746365 | # pLL promoter - BBa_I746365 | ||
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# LuxR Translational unit | # LuxR Translational unit | ||
| - | = | + | <p><span style="font-weight:bold; font-size:150%; color:#FF6600;">OUR PARTS</span></p> |
| + | |||
# YcdB | # YcdB | ||
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| - | = | + | <p><span style="font-weight:bold; font-size:150%; color:#FF6600;">Assembly</span></p> |
| + | |||
# Const. Promoter-LacO | # Const. Promoter-LacO | ||
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| - | |||
| - | == | + | |
| + | <p><span style="font-weight:bold; font-size:175%; color:#FF6600;">Failed Transformations</span></p> | ||
| + | |||
| + | |||
| + | |||
| + | <p><span style="font-weight:bold; font-size:150; color:#FF6600;">Backbones</span></p> | ||
| + | |||
# pSB1AK3 with CcdB gene | # pSB1AK3 with CcdB gene | ||
# pSB1T3 with CcdB gene | # pSB1T3 with CcdB gene | ||
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# pSB1AC3 with mRFP | # pSB1AC3 with mRFP | ||
| - | = | + | <p><span style="font-weight:bold; font-size:150; color:#FF6600;">Native Biobricks</span></p> |
| + | |||
| + | |||
# Lysis Cassette | # Lysis Cassette | ||
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# COnstitutive CFP | # COnstitutive CFP | ||
| - | = | + | <p><span style="font-weight:bold; font-size:150; color:#FF6600;">GEL PICTURES</span></p> |
| - | [[Image:10sepgel.jpg|center]] | + | |
| + | [[Image:10sepgel.jpg|center|500px]] | ||
| + | <br><br> | ||
| + | |||
[[Image:14octgel.jpg|center|500px]] | [[Image:14octgel.jpg|center|500px]] | ||
| + | <html> | ||
| + | <table border="0" align="center"> | ||
| + | <tr><td>Lane1</td><td>Lane2</td><td>Lane3</td> <td>Lane4</td><td>Lane5</td><td>Lane6</td><td>Lane7</td></tr> | ||
| + | <tr><td>TorA</td><td>YcdB</td><td>YcdB</td><td>LacO</td><td>LacO</td><td>pSB1AK3</td><td>pSB1AK3</td></tr></table> | ||
Latest revision as of 02:44, 22 October 2009
- Home
- Team
- Project
Summary Details Results Modeling
- Related
- Safety
- Parts
- Notebook
Characterization of And Gates:
Even as the wiki freezes we are characterizing Const.Prom-LacO-mRFP for its expression. We hope we get positive results.
We carried out the following transformations successfully:
Native Biobricks
- pLL promoter - BBa_I746365
- GFP coding sequence - BBa_E0040
- RFP coding sequence
- GFP Translational unit
- RFP Translational unit
- Enhanced CFP
- Fast GFP
- Constitutive promoter -BBa_J23102
- Promoter + RBS
- T7 promoter
- pLuxR
- Double terminator
- RBS
- Constitutive LuxI
- LuxR Translational unit
OUR PARTS
- YcdB
- TorA
- Lac Operator
Assembly
- Const. Promoter-LacO
- PluxR- LuxI
- {Promoter + RBS}-GFP
- {Promoter + RBS}-TorA
- {Promoter + RBS}-Ycdb
- luxR-PluxR-{RBS-LuxI}
- Promoter + RBS
- YcdB-GFP
- TorA-GFP
- {Promoter + RBS}-LuxR
- {Promoter + RBS}-YcdB-GFP
- {Promoter + RBS}-TorA-GFP
- Const. Promoter-LuxR
Failed Transformations
Backbones
- pSB1AK3 with CcdB gene
- pSB1T3 with CcdB gene
- pSB1C3 with CcdB gene
- pSB1K3 with CcdB gene
- pSB1A3 with CcdB gene
- pSB1AC3 with CcdB gene
- pSB1AK3 with mRFP
- pSB1T3 with mRFP
- pSB1C3 with mRFP
- pSB1K3 with mRFP
- pSB1A3 with mRFP
- pSB1AC3 with mRFP
Native Biobricks
- Lysis Cassette
- Constitutive GFP
- COnstitutive CFP
GEL PICTURES
Lane1 Lane2 Lane3 Lane4 Lane5 Lane6 Lane7 TorA YcdB YcdB LacO LacO pSB1AK3 pSB1AK3
